Alpha 1-proteinase inhibitor and resistance to acute blood loss during injection of beta-1,3-carboxymethylglucan.

The present study was undertaken to evaluate the mechanism of protective effect of semisoluble alpha-1,3-carboxymethylglucan during massive acute hemorrhage. CBA mice were injected i.v. with glucan (25 mg/kg) 24 h prior to hemorrhage (50% of blood circulating volume). Survival data indicated that glucan increased survival compared with the control. However alpha 1-proteinase inhibitor activity in serum has been even decreased 24 h after glucan administration. Moreover enhancement of active oxygen form production and leakage of cytosolic and lysosomal enzymes after alpha-1,3-carboxymethylglucan application was noted.