[Establishment of a rabbit model of Acanthamoeba keratitis].

OBJECTIVE

To establish an animal model of Acanthamoeba keratitis.

METHODS

Six New Zealand white rabbits were each injected intrastromally with Acanthamoeba suspension 3 days after subconjunctival injection with dexamethasone.

RESULTS

All of the 6 rabbits developed keratitis. Acanthamoeba protozoa were identified by the methods of corneal scraping with 10% potassium hydroxide wet mount examined under microscope, corneal protozoa culture and pathological section examination.

CONCLUSIONS

A rabbit model of Acanthamoeba keratitis was established.