Hemangioendothelial sarcoma in brown adipose tissue of mouse induced by carcinogenic heterocyclic amine, Glu-P-1.

2-Amino-6-methyldipyrido[1,2-a:3',2'-d]limidazole, a potent bacterial mutagen from glutamic acid pyrolysate, when given to male CDF1 mice at a concentration of 0.05% in the diet induced blood vessel tumors characterized histopathologically. These hemangioendothelial sarcomas showed positive alkaline phosphatase activity histochemically and Weibel-Palade bodies ultrastructurally. Neoplasms were predilective for brown adipose tissue and foci of atypical cells, not peliosis-like lesions, appear to be an early stage in tumor development.