[Identification and nucleotide polymorphisms in Brassica rapa genes coding cold shock domain proteins (CSDP)].

Full-length BrCSDP2 and BrCSDP4 cold shock gene sequences of Brassica rapa are obtained. It is shown that the isolated genes belong to a group AtCSP2/AtCSP4 of Arabidopsis thaliana and TsCSDP2/TsCSDP4 of Thellungiella salsuginea genes encoding proteins with a cold shock domain (CSD) and two zinc finger motives. The structure and the allelic variants of these genes are described and characterized. It is shown that the identified allelic polymorphism is due to both of point substitutions and small indels. Coefficients of total genetic similarity ranged from 1.0 to 0.53. In tern the genetic similarity coefficient for BrCSDP2 and AtCSDP2 was 0.89, and for BrCSDP4 and AtCSDP4 was 0.85.Translation in silico of gene sequences has revealed amino acid substitutions in the protein sequence, but no significant correlation between the detected polymorphism and signs of resistance to cold stress were found.