[The secondary metabolites of the HS-3 Alternaria sp. fungus associated with holothurians].

OBJECTIVE

The metabolites of HS-3 associated with holothurians were studied, which was identified by molecular biology as Alternaria sp..

METHODS

The holothurians were gathered from the Sea of Zhifu Islet, Shandong Province. HS-3 Alternaria sp. was culternitived in potato medium, and four compound was got by TLC, chromatography and HPLC, and 1-hydroxyl-3-methylanthracene-9,10-dione (1), chrysophanol (2), sterigmatocystin (3) and cerebroside (4) were elucated by modern spectrum.

CONCLUSIONS

All of this provides scientific data for further study of holothurians, and the four coumpouns are isolated from the microbe associated with holothurians for the first time.