Tumorigenesis inheritance from the putative progenitor species of Nicotiana rustica.

Hybrid seedlings from crosses of Nicotiana rustica×N. langsdorffii and N. rustica×N. alata show tumors including teratomas and vitrification. In the present study, we attempted to elucidate the genetic background leading to tumorigenesis and vitrification from the viewpoint of the amphidiploidy of N. rustica. The species N. undulata, N. paniculata, and N. knightiana have been suggested to be the progenitors of N. rustica or closely related to its progenitors. We tested tumorigenesis in interspecific hybrids between these putative progenitors of N. rustica and N. langsdorffii or N. alata, which are the species in section Alatae. The hybrid seedlings were cultured in test tubes and their morphological characteristics were observed. According to previous reports, most of the hybrid seedlings from the crosses N. rustica×N. langsdorffii and N. rustica×N. alata formed tumors and showed vitrification. In crosses with every putative progenitor of N. rustica, a portion of hybrid seedlings formed tumors and showed vitrification. These observations suggested that N. rustica inherited the factors leading to expression of abnormal symptoms from its putative progenitors. We also observed the influence of high temperature on the expression of abnormal symptoms of hybrid seedlings from the cross N. rustica×N. alata. While these hybrids developed teratomas and other tumors at 28°C, when cultured at 34°C, they did not show any abnormalities. This is the first report to show that phenotypic abnormalities in hybrid seedlings of N. rustica×N. alata are temperature sensitive.