Green synthesis of silver nanoparticle using Leonotis nepetifolia and their toxicity against vector mosquitoes of Aedes aegypti and Culex quinquefasciatus and agricultural pests of Spodoptera litura and Helicoverpa armigera

Pest insects causing damage to cultivable crops and food products by feeding, fecundity, and parasitizing livestock, also being a nuisance to human health. In consideration with human health, the World Health Organization reports that more than 50% of the world's population is presently at risk from mosquito-borne diseases. Mosquitoes are primary vectors for major dreadful diseases such as yellow fever, malaria, and dengue fever, which infect millions of human beings all over the world and kill millions of peoples every year. The present research work was carried out to evaluate the antifeedant, larvicidal, pupicidal, larval, and pupal duration activity of Leonotis nepetifolia-mediated silver nanoparticles (AgNPs) against Spodoptera litura, Helicoverpa armigera, Aedes aegypti, and Culex quinquefasciatus. Biosynthesized AgNPs were characterized through various techniques such as UV-Vis spectrometer, X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential analysis. The AgNPs showed potential antifeedant activity of 78.77% and 82.16% against the larvae of S. litura and H. armigera, respectively. The maximum larval mortality rate (78.49% and 72.70%) and maximum pupal mortality rate (84.66% and 77.44%) were observed against S. litura and H. armigera. Mosquito larvae were tested with biosynthesized AgNPs, and recorded LC₅₀ values were 47.44 ppm and 35.48 ppm on A. aegypti and C. quinquefasciatus, respectively. The histological examinations showed that the acceleration of the nanomaterial caused severe tissue damage in the epithelial and goblet cells in the larval midgut region of S. litura, H. armigera, A. aegypti, and C. quinquefasciatus. Biosynthesis of silver nanoparticles using L. nepetifolia is an ideal eco-friendly approach for the management of insect pests. Graphical abstract.

Keywords: Aedes aegypti; Culex quinquefasciatus; Helicoverpa armigera; Leonotis nepetifolia; Silver nanoparticles; Spodoptera litura.