Radioiodinated Ginger Compounds (6-gingerol and 6-shogaol) and Incorporation Assays on Breast Cancer Cells.

6-Gingerol (6G) and 6-Shogaol (6S) are main active components of ginger. 6-Gingerol is known with its anti-metastatic and anti-invasive pharmacological activities on cancer cells, also 6-Shogaol inhibits breast cancer cell invasion.In this study, radioiodination (131I) of 6G and 6S was aimed. Additionally, it's aimed to monitor their incorporation behavior on breast cancer cell lines.6-Gingerol was isolated from the fresh ginger-roots extract, additionally, dehydrated to obtain 6-Shogaol. 6G and 6S were radioiodinated using iodogen method. Quality control studies of radioiodinated ginger compounds (6G and 6S) were performed by thin layer radio-chromatography. In vitro studies of radioiodinated ginger compounds on MCF-7 and MDA-MB-231 cells were performed with incorporation assays.6-Gingerol and 6-Shogaol were radioiodinated (131I-6G and 131I-6S) in high yields over 95%. 131I-6S demonstrated higher incorporation values than 131I-6G on MDA-MB-231 cells. Incorporation behavior of 131I-6G and 131I-6S were similar on MCF-7 cells.It's observed that ginger compounds were radioiodinated successfully and 131I-6S have a noteworthy incorporation on MDA-MB-231 cells which is a known breast carcinoma cell line with highly invasive characteristics.