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Assessment of chitosan biopolymer encapsulated α-Terpineol against fungal, aflatoxin B1 (AFB1) and free radicals mediated deterioration of stored maize and possible mode of action.

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This study reports enhanced efficacy of encapsulated α-Terpineol to control fungal, aflatoxin B₁ (AFB₁) and free radicals mediated deterioration of stored maize samples. The α-Terpineol loaded chitosan nanoemulsion (α-TCsNe) was characterized through SEM, FTIR and XRD

Ergosterol accumulation and oil quality changes in stored soybean invaded by Aspergillus ruber (A. glaucus group).

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Soybean seed samples inoculated with spores of Aspergillus ruber were stored for 20 to 140 days at 25 +/- 1 degrees C with moisture content varying from 11.3% to 17.7%. Seeds were colonised by the fungus within 20 days at all moisture levels. Ergosterol concentration in seeds increased with time of

UV induced conversion during drying of ergosterol to vitamin D in various mushrooms: Effect of different drying conditions

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Background: Mushrooms are increasingly popular around the world as a nutritional food which is an excellent source of vitamin D₂. Although natural mushrooms often contain very little vitamin D₂ as many are grown in

Cloning and characterization of the lanosterol 14alpha-demethylase (ERG11) gene in Cryptococcus neoformans.

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The ergosterol pathway in fungal pathogens is an attractive antimicrobial target because it is unique from the major sterol (cholesterol) producing pathway in humans. Lanosterol 14alpha-demethylase is the target for a major class of antifungals, the azoles. In this study we have isolated the gene

Fluorometric detection and estimation of fungal biomass on cultural heritage materials.

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A wide variety of cultural heritage materials are susceptible to fungal deterioration. The paper, canvas, and stone constituents of our cultural heritage are subjected to harmful physical and chemical processes as they are slowly consumed by fungi. Remediation of fungal contamination can be costly

Effect of two monoterpene phenols on antioxidant defense system in Candida albicans.

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Thymol and carvacrol from the class of monoterpene phenols are one of the most potent plant essential oil components possessing antimicrobial effects. Known for their wide bioactive spectrum, these positional isomers of isopropyl cresol deplete ergosterol content, compromise membrane permeability,

Cloning and analysis of Trypanosoma cruzi lanosterol 14alpha-demethylase.

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Trypanosoma cruzi infection, transmitted by insect vectors or blood transfusions, is an important cause of morbidity and mortality in many Latin American countries. Treatments are toxic and frequently ineffective in curing patients with chronic manifestations of the infection (Chagas disease).

Anti-microbial and anti-mycotoxigenic activities of endophytic Alternaria alternata isolated from Catharanthus roseus (L.) G. Don.: molecular characterisation and bioactive compound isolation.

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The present study investigated the anti-microbial and anti-mycotoxigenic activities of the ethyl acetate extract (EA) and a bioactive compound obtained from an endophytic fungus Alternaria alternata isolated from Catharanthus roseus leaves. A. alternata was identified using

Contamination of wheat grain with microscopic fungi and their metabolites in Poland in 2006-2009.

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Microscopic fungi are microorganisms commonly found in cereal products. Pathogens of cereals colonising kernels are responsible, among other things, for deterioration of the technological value of grain. However, the greatest threat is posed by mycotoxins produced by toxin-forming strains of these

Application of monoclonal antibodies in quantifying fungal growth dynamics during aerobic spoilage of silage.

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Proliferation of filamentous fungi following ingress of oxygen to silage is an important cause of dry matter losses, resulting in significant waste. In addition, the production of mycotoxins by some filamentous fungi poses a risk to animal health through mycotoxicosis. Quantitative assessment of

The impact of medium acidity on the chronological life span of Saccharomyces cerevisiae - lipids, signaling cascades, mitochondrial and vacuolar functions.

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Because of its multifactorial nature, aging is one of the most complicated cell phenomena known. A systems biology approach, which aims to understand the organism as a whole rather than concentrate on the behaviors of individual genes, thus comprises a seamless tool for investigating the aging

Novelties in the multifaceted miconazole effects on skin disorders.

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BACKGROUND Miconazole nitrate is a time-honored antifungal of the imidazole class. OBJECTIVE To revisit the various aspects of action of the drug in a dermatologic setting. METHODS Review of the current peer-reviewed publications. CONCLUSIONS Miconazole essentially inhibits 14alpha-demethylase, an

Physiological and transcriptional responses of Saccharomyces cerevisiae to d-limonene show changes to the cell wall but not to the plasma membrane.

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Monoterpenes can, upon hydrogenation, be used as light-fraction components of sustainable aviation fuels. Fermentative production of monoterpenes in engineered microorganisms, such as Saccharomyces cerevisiae, has gained attention as a potential route to deliver these next-generation fuels from

Biochemical targets for antifungal azole derivatives: hypothesis on the mode of action.

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The selective interaction of low concentrations of azole derivatives and other nitrogen heterocycles with cytochrome P-450 may be at the origin of the inhibition of ergosterol biosynthesis. From the depletion of ergosterol and the concomitant accumulation of 14 alpha-methylsterols, alterations in

Antimicrobial activity, antiaflatoxigenic potential and in situ efficacy of novel formulation comprising of Apium graveolens essential oil and its major component.

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The present study reports the formulation of Apium graveolens essential oil (AGEO) with its major components linalyl acetate (LA) and geranyl acetate (GA) (1:1:1) as a novel green preservative for protection of postharvest food commodities from fungal infestations, aflatoxin B₁

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