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UDP-arabinopyranose mutase gene expressions are required for the biosynthesis of the arabinose side chain of both pectin and arabinoxyloglucan, and normal leaf expansion in Nicotiana tabacum.

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Plant cell walls are composed of polysaccharides such as cellulose, hemicelluloses, and pectins, whose location and function differ depending on plant type. Arabinose is a constituent of many different cell wall components, including pectic rhamnogalacturonan I (RG-I) and II (RG-II),

A high proportion of hybridomas raised to a plant extract secrete antibody to arabinose or galactose.

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A high proportion of hybridomas, obtained from mice immunized with style extracts prepared from mature flowers of an ornamental tobacco, Nicotiana alata, secrete antibody to arabinogalactan protein (AGP). The specificity of the antibodies secreted by three cloned cell lines is primarily directed to

Composition of the cell walls of Nicotiana alata Link et Otto pollen tubes.

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Cell walls isolated from pollen of Nicotiana alata germinated in vitro contain glucose and arabinose as the predominant monosaccharides. Methylation analysis and cytochemical studies are consistent with the major polysaccharides being a (1→3)-β-D-glucan (callose) and an arabinan together with small

A galactose-rich, cell-wall glycoprotein from styles of Nicotiana alata.

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A basic, galactose-rich style glycoprotein (GaRSGP) encoded by a previously characterized style-specific cDNA (NaPRP4) has been isolated from the styles of Nicotiana alata and structurally characterized. The glycoprotein is associated with cell walls in the transmitting tract and is composed of

Wildfire of Soybean Caused by Pseudomonas syringae pv. tabaci, a New Disease in Korea.

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In 2006 and 2007, a new bacterial disease was observed in field-cultivated soybeans in Boeun District and Munkyung City of Korea. The disease caused severe blighting of soybean (Glycine max) leaves. Soybean leaves in fields showed yellowish spots with brown centers. Brown and dead areas of variable

Purification and characterization of a galactose-rich basic glycoprotein in tobacco.

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We found a galactose-rich basic glycoprotein (GBGP) in the cell walls of cultured tobacco (Nicotiana tabacum) cells. GBGP and extensin were isolated as the major components of basic, salt-extracted cell wall glycoproteins. GBGP and extensin were separated by gel filtration in 6 M guanidine

Metabolism of myo-Inositol and growth in various sugars of suspension-cultured tobacco cells.

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Tobacco (Nicotiana tabacum L.) cells were cultured in a liquid medium which contained sucrose as a source of carbon and energy. Various cell-wall constituents and wall precursors (L-arabinose, D-xylose, D-galactose, D-mannose, D-glucuronate, myo-inositol) were added to cells growing in this medium

Interaction of a hydroxyproline-rich glycoprotein from tobacco callus with potential pathogens.

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A hydroxyproline-rich glycoprotein was isolated from tobacco (Nicotiana tabacum L.) callus tissue cultures by an acidic-ethanol extraction procedure and purified to about 95% homogeneity by ion exchange chromatography on carboxymethyl cellulose. This glycoprotein agglutinated cells of an avirulent

Pollen tube cell walls of wild and domesticated tomatoes contain arabinosylated and fucosylated xyloglucan.

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OBJECTIVE In flowering plants, fertilization relies on the delivery of the sperm cells carried by the pollen tube to the ovule. During the tip growth of the pollen tube, proper assembly of the cell wall polymers is required to maintain the mechanical properties of the cell wall. Xyloglucan (XyG) is

First Report of Bacterial Bark Canker of Walnut Caused by Brenneria nigrifluens in Hungary.

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During August 2012, vertical oozing cankers were sporadically observed on trunks and branches of walnut trees (Juglans regia) in the city of Zánka, near Lake Balaton and other parts of Hungary including Budapest, Győr, and Tatabánya cities. Cankers were observed on trunks and branches where

First Report of Bacterial Wilt on Chrysanthemum Caused by Dickeya chrysanthemi (syn. Erwinia chrysanthemi) in Hungary.

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Chrysanthemum (Chrysanthemum spp.) is a popular potted and cut plant ornamental in Hungary. In September 2012, chrysanthemum plants (Chrysanthemum morifolium Ramat. cv. Palisade) showing wilt symptoms were collected from different greenhouses in the cities of Budakalász and Pilis near Budapest.

Immunoaffinity purification and biochemical characterization of plasma membrane arabino-galactan-rich glycoproteins of Nicotiana glutinosa.

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Monoclonal antibody PN 16.4B4 reacts with an epitope present on the external face of the plasma membrane as shown by immunofluorescent staining of Nicotiana glutinosa L. protoplasts (Norman et al. 1986, Planta 167, 452-459). We show here that this epitope is present in a glycan moiety and defines a

Class III pistil-specific extensin-like proteins from tobacco have characteristics of arabinogalactan proteins.

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Class III pistil-specific extensin-like proteins (PELPIII) are specifically localized in the intercellular matrix of tobacco (Nicotiana tabacum) styles. After pollination the majority of PELPIII are translocated into the callosic layer and the callose plugs of the pollen tubes, which could suggest a

Characterization of a family of Arabidopsis genes related to xyloglucan fucosyltransferase1.

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To understand primary cell wall assembly in Arabidopsis, we have focused on identifying and characterizing enzymes involved in xyloglucan biosynthesis. Nine genes (AtFUT2-10) were identified that share between 47% and 62% amino acid similarity with the xyloglucan-specific fucosyltransferase AtFUT1.

Cell Walls of Tobacco Cells and Changes in Composition Associated with Reduced Growth upon Adaptation to Water and Saline Stress.

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The relative mass of the cell walls of tobacco (Nicotiana tabacum L.) cells adapted to grow in medium containing 30% polyethylene glycol 8000 or 428 millimolar NaCl was reduced to about 50% of that of the walls of unadapted cells. Cellulose synthesis was inhibited substantially in adapted cells. The

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