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Evaluation of -propiolactone-inactivated- and chloroform-treated-virus vaccines against bovine viral diarrhea-mucosal disease.

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Antidiarrheal and antinociceptive activities of ethanol extract and its chloroform and pet ether fraction of Phrynium imbricatum (Roxb.) leaves in mice.

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BACKGROUND The objective of the study was to evaluate the antidiarrheal and antinociceptive activities of ethanol extract and its chloroform and pet ether fraction of Phrynium imbricatum (Roxb.) leaves in mice. METHODS In the present study, the dried leaves of P. imbricatum were subjected to

Physicochemical properties of Nebraska calf diarrhea virus hemagglutinin.

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Highly purified Nebraska calf diarrhea virus (NCDV) was prepared by cesium chloride density gradient centrifugation. The effect of temperature, pH, different concentrations of formaldehyde, chloroform, ether, ethyl alcohol, and methyl alcohol on NCDV hemagglutinin and virus morphology was studied.

[Molecular epidemiology of Cryptosporidium infection in infants with diarrhea in Wuhan City].

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OBJECTIVE To understand the prevalence of Cryptosporidium infection in diarrhea infants under 2 years old in Wuhan City, so as to provide the epidemiological evidence for the prevention and treatment of cryptosporidiosis. METHODS The fecal samples from infants under 2 years old with diarrhea were

A survey of the frequency of cytomegalovirus-associated diarrhea in immunocompromised patients using a non-invasive method.

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UNASSIGNED Cytomegalovirus (CMV) infection is the most common viral opportunistic infection causing gastrointestinal diseases such diarrhea and colitis in immunocompromised patients. The development and performance of a robust and sensitive PCR assay are usually evaluated to detect CMV DNA in human

Development of a PCR amplification assay as a screening test using bulk milk samples for identifying dairy herds infected with bovine viral diarrhea virus.

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The approach of cDNA synthesis followed by polymerase chain reaction (PCR) amplification was used to develop a rapid screening test for the detection of bovine viral diarrhea virus (BVDV) in bulk tank milk samples. The initial development of this detection method was done using lactating Holstein

Tween 80: a marker for differentiation of hog cholera and bovine viral diarrhea viruses.

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Markers for differentiating hog cholera and bovine viral diarrhea viruses were studied using Tween 80, chloroform, trichlorotrifluoroethane and tri (n-butyl) phosphate. Attenuated A and virulent Ames strains of hog cholera virus were employed. Moreover, the NADL PK-15 cell culture adopted strain and

Physiochemical characterization of a neonatal calf diarrhea virus.

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Physicochemical characteristics of two isolates of a neonatal calf diarrhea virus were investigated. Neither isolate was sensitive to ether or chloroform, both were stable at pH 3.0, were relatively heat resistant, but were thermolabile when heated to 50 degrees C for one hour in the presence of 1.0

Antibacterial Activities, Phytochemical Screening and Metal Analysis of Medicinal Plants: Traditional Recipes Used against Diarrhea.

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The aim of this study was to explore the phytochemical composition, heavy metals analysis and the antibacterial activity of six medicinal plants i.e., Terminalia chebula Retz (fruits), Aegle marmelos L., (fruits), Curcuma longa L., (rhizomes), Syzygium aromaticum L.,

The anti-inflammatory and antinociceptive properties of the chloroform fraction from Phyllanthus niruri plant is mediated via the peripheral nervous system.

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Phyllanthus niruri (Euphorbiaceae) is used folklorically for the treatment of diabetes, malaria, fever, diarrhea, liver disease, and urolithiasis. As an initial step toward isolating compounds effective against inflammation and pain, this study is aimed at providing scientific evidence for the

Detection of picobirnavirus in HIV-infected patients with diarrhea in Argentina.

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Diarrhea due to enteric pathogens is an important complication of advanced HIV infection. Picobirnaviruses are agents recently linked with human enteritis. In total, 197 fecal samples collected from HIV-infected and noninfected patients with and without diarrhea were investigated for the presence of

The use of Euphorbia hirta L. (Euphorbiaceae) in diarrhea and constipation involves calcium antagonism and cholinergic mechanisms.

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Euphorbia hirta (Linn) family Euphorbiaceae has been used in indigenous system of medicine for the treatment of gastrointestinal disorders. This study was designed to determine the pharmacological basis for the medicinal use of E. hirta in diarrhea and

Control of coliform bacteria detected from diarrhea associated patients by extracts of Moringa oleifera.

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The aims of this study were to determine the total population of coliform bacteria in the samples collected from diarrhea associated patients from the local area of Bangladesh and to examine the antibacterial efficacy of leaf extracts of Moringa oleifera (Moringaceae) against the isolated coliform

Purification and characterization of epizootic diarrhea of infant mice virus.

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Epizootic diarrhea of infant mice virus has been purified from the intestines of infected mice using enzymatic digestion, precipitation with polyethylene glycol, extraction with Genesolv-D, and sucrose step-gradient centrifugation. The purified virus was found to be stable at -70 C or when treated

[Detection of the genome of bovine viral diarrhea virus (BVDV) using the polymerase chain reaction after reverse transcription (RT-PCR): comparison of methods for the isolation of ribonucleic acid (RNA) from clinical samples].

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The RT-PCR is an in vitro technique that is increasingly being used for diagnosis of viral animal pathogens. Due to its high sensitivity it is considered as an alternative to current standard methods for detecting BVDV especially in pooled samples, e.g. from bulk tank milk. A prerequisite for the

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