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Morphological response and survival of hepatoma cells during fractionated hyperthermia: effect of glycerol.

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Reuber H35 rat hepatoma cells rounded and became spherical during hyperthermia at 42.5 degrees C. When returned to 37 degrees C, the cells recovered and spread out again. As soon as the cells had recovered from the morphologically expressed stress, they expressed tolerance to a second hyperthermia

Colorado tick fever virus in cell culture. II. Physical and chemical properties.

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Trent, Dennis W. (University of Oklahoma School of Medicine, Oklahoma City), and L. Vernon Scott. Colorado tick fever virus in cell culture. II. Physical and chemical properties. J. Bacteriol. 91:1282-1288. 1966.-Heat-inactivation kinetics for Colorado tick fever (CTF) virus grown in L cells

The relationship of increased nuclear protein content induced by hyperthermia to killing of HeLa S3 cells.

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The role of a heat-induced increase in nuclear protein mass in killing of cells by hyperthermia was investigated jointly by two groups that had previously reported apparently conflicting results. A correlation between the fraction of HeLa S3 cells killed and the protein content of nuclei isolated

The Aquaporin gene family of the yellow fever mosquito, Aedes aegypti.

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BACKGROUND The mosquito, Aedes aegypti, is the principal vector of the Dengue and yellow fever viruses. During feeding, an adult female can take up more than its own body weight in vertebrate blood. After a blood meal females excrete large amounts of urine through their excretion system, the

Comparison of the survival on ice of thawed Theileria parva sporozoites of different stocks cryoprotected by glycerol or sucrose.

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Stabilates of Theileria parva sporozoites are mostly delivered in liquid nitrogen tanks to the East Coast fever immunization points. Using an in vitro titration model, we assessed the loss of infectivity of several stabilates when they are stored in ice baths for up to 24 h. Comparisons, with

In vitro application of paclitaxel loaded magnetoliposomes for combined chemotherapy and hyperthermia.

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Paclitaxel loaded thermosensitive magnetoliposomes containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-glycerol (PG) were prepared by thin film hydration method. Encapsulation efficiencies of paclitaxel and citric acid coated Fe(3)O(4)

Effects of hyperthermia and membrane-active compounds or low pH on the membrane fluidity of Chinese hamster ovary cells.

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Chinese hamster ovary (CHO) cells heated in the presence of the membrane-active agents procaine, ethanol, butylated hydroxytoluene (BHT) and glycerol were analysed for changes in fluorescence polarization of the lipid probe (1-[4(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene, an indicator of

Heat protection by glycerol in vitro.

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Heating of either Chinese hamster ovary or HeLa cells in medium containing glycerol protected against thermal killing. Above glycerol concentrations of 100 mM, protection of Chinese hamster ovary cells increased in a concentration-dependent manner. Exposure to glycerol only, before or after heating

Effect of glycerol and low pH on heat-induced cell killing and loss of cellular DNA polymerase activities in Chinese hamster ovary cells.

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A whole-cell assay technique for DNA polymerase alpha and beta was used to measure the activities of both enzymes in Chinese hamster ovary (CHO) cells after hyperthermic treatment of 42.2 - 45.5 degrees C in acidic or basic environment and in the presence or absence of 5% glycerol. Cell survival was

Glycerol restores heat-induced p53-dependent apoptosis of human glioblastoma cells bearing mutant p53.

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BACKGROUND We have previously reported that glycerol acts as a chemical chaperone to restore the expression of WAF1 in some human cancer cell lines bearing mutant p53. Since the expression of WAF1 is up-regulated by activated wildtype p53, glycerol appears to restore wtp53 function. The aim of the

In vitro localized release of thermosensitive liposomes with ultrasound-induced hyperthermia.

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Localized drug delivery with ultrasound-induced hyperthermia can enhance the therapeutic index of chemotherapeutic drugs by improving efficacy and reducing systemic toxicity. A novel in vitro method for the activation of drug-loaded thermosensitive liposomes is described. In particular, a

RNA-dependent RNA polymerase activity of Classical swine fever virus NS5B protein expressed in natural host cells.

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The NS5B gene, cloned from Classical swine fever virus (CSFV) genome, was expressed in porcine kidney cells PK-15, natural host of CSFV. In purifying cytoplasmic extracts from these cells by means of different concentrations of salt, glycerol and detergent four fractions, namely crude supernatant

The effect of sample degradation and RNA stabilization on classical swine fever virus RT-PCR and ELISA methods.

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Classical swine fever (CSF), also known as hog cholera, is a highly contagious viral infection of swine caused by a member of the genus pestivirus of the family, Flaviviridae. The need for accurate laboratory diagnosis of CSF is particularly important as it is more reliable than clinical diagnosis.

Maximal exercise and muscle energy metabolism after recovery from exercise hyperthermia syndrome.

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Muscle energy metabolism was studied in 30 subjects after recovery from exercise hyperthermia syndrome (EHTS subjects) and 15 healthy men with identical physical activities. Blood lactate, free fatty acid (FFA), serum creatine kinase activity (CK), and glycerol and the temperature in the auditory

Effects of graded exercise on leg exchange of energy substrates in malignant hyperthermia susceptible subjects.

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It has been speculated that, in malignant hyperthermia-susceptible (MHS) individuals, an abnormality of sympathetic activity is seen during stressful situations, such as exercise. The authors investigated whether muscle metabolism in eight MHS subjects, at rest and during moderate and heavy

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