Patients diagnosed as suffering from Fibromyalgia according to the ACR 1990 criteria

Patients diagnosed as suffering from Systemic Lupus Erythematosus by the ACR criteria

Fibromyalgia

Systemic Lupus Erythematosus

Tel Aviv Sourasky Medical Center Institute of Rheumatlogy

Membrane Polyunsaturated Fatty Acid Content in Fibromyalgia and Systemic Lupus Erythematosus (SLE)

Association of Membrane Polyunsaturated Fatty Acid Content and Intracellular Magnesium Concentration With Mental and Physical Symptoms in Fibromyalgia and SLE: a Case-control Study

5 mls of blood centrifuged at 4000 rpm for 10 min at 5 o C, removal of plasma and buffy coat; addition of 5 ml cold saline + EDTA ; centrifuge at 3000 rpm for 10 min at 5oC; repeat; flushing of erythrocytes with N2; storage at -70o C . Lipid extraction by homogenization in hexane/ isopropanol. Fatty acid analysis: fatty acids will be converted to methyl esters by heating with BF3 in methanol, and the methyl esters separated on a HP 5890 Series II Gas Chromatograph containing a flame ionization detector..

hexane/إعياء

Membrane Polyunsaturated Fatty Acid Content in Fibromyalgia and Systemic Lupus Erythematosus (SLE)

قاعدة بيانات الأعشاب الطبية الأكثر اكتمالا التي يدعمها العلم