[Physico-chemical properties and activity of glycoconjugate LbGp2 from Lycium barbarum L].
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OBJECTIVE
To isolate and purify a glycoconjugate (LbGp2) from the fruit of Lycium barbarum L. and study its immunoactivity and antioxidative activity.
METHODS
By means of gel permeation chromatography, LbGp2 was purified. Based on HPLC, CE, GC, SEC and component analysis and so on, its physico-chemical properties were studied.
RESULTS
Molecular weights of LbGp2 was 68.2 ku and its carbohydrate content was up to 90.7%. Component analysis showed that it composed of Ara and Gal in a molar ratio of 3:4, and 18 kinds of amino acids. The immunologic function and bioactivity of Lbp2 has been studied preliminarily. Lbp2 was shown to increase rate of phagocyticaction and phagocytic index, promote lymphocyte translation and accelerate the production of serum hemolysin. LbGp2 has distinct effect of antioxidation and the superoxide anion produced by DMSO-NaOH system was scavenged effectively.
CONCLUSIONS
LbGp2 was shown to be a kind of homogeneous glycoconjugate with good immunoactivity and antioxidative activity.
