[Purification of total flavonoids from the extracts of Thesium Chinese with macroporous adsorption resin].
Ключавыя словы
Рэферат
OBJECTIVE
To optimize the process parameters for purifying total flavonoids from Thesium chinese with D101 macroporous adsorption resin.
METHODS
Purification technology of total flavonoids from the extracts of Thesium chinese was investigated by dynamic adsorption method with the transfer rates and the purity of total flavonoids as indexes.
RESULTS
The optimal purifying conditions were as follows: sample concentration was 2.259 mg/mL with a speed of 1 BV/h, eluent concentration was 70% alcohol with a speed of 1BV/h. After purification, the transfer rate of total flavonoids was 94.44% and the purity of total flavonoids achieved 12.45%, which was 4 times of that by coarse extraction (2.91%).
CONCLUSIONS
The purification technology is simple, stable and can significantly improve the contents of total flavoids in extracts.
