Could curcumin ameliorate titanium dioxide nanoparticles effect on the heart? A histopathological, immunohistochemical, and genotoxic study.

The evaluation of the toxicological effects of titanium dioxide nanoparticles (TiO₂NPs) is increasingly important due to their growing occupational and industrial use. Curcumin is a yellow curry spice with a long history of use in herbal medicine and has numerous protective potentials such as antioxidant, antimicrobial, anti-inflammatory, and anti-apoptotic effects. Accordingly, we tested the hypothesis that curcumin could ameliorate TiO₂NP-induced cardiotoxic and genotoxic effects in adult male albino rats. For this purpose, 48 adult male albino rats were randomized into five groups; all treatment was by oral gavage once daily for 90 days: group I (8 rats), untreated control; group II (16 rats), subdivided into vehicle control IIa (8 rats) received saline and vehicle control IIb (8 rats) received corn oil; group III (8 rats) orally gavaged with curcumin dissolved in 0.5 ml corn oil at a dose of 200 mg/kg b.w./day; group IV treated with TiO₂NPs at a dose of 1200 mg/kg b.w./day (1/10 LD₅₀) suspended in 1 ml of 0.9% saline; group V treated with curcumin + TiO₂NPs (the same previously mentioned doses). Curcumin was orally gavaged for 7 days before TiO₂NPs treatment was initiated, and then they received TiO₂NPs along with curcumin at the same doses for 90 days. TiO₂NPs administration resulted in several myocardial cytomorphic changes as structurally disorganized, degenerated, and apoptotic cardiomyocytes and the newly implemented 3-nitrotyrosine immune expression rendered strong evidence that these effects derived from the cardio myocellular oxidative burden. Furthermore, comet assay results confirmed TiO₂NP-related DNA damage. Remarkably, all these changes are partially mitigated in rats treated with both curcumin and TiO₂NPs. Our results suggest that concurrent curcumin treatment has a beneficial role in ameliorating TiO₂NP-induced cardiotoxicity and this may be mediated by its antioxidative property.