[Effects of oxidized LDL, hypoxia, and 5-hydroxytryptamine on 5-HT2A receptor and intracellular free Ca2+ in cultured vascular smooth muscle cells].
Ключови думи
Резюме
OBJECTIVE
To investigate the mechanisms of this hyperreactivity, the effects of oxLDL, hypoxia, and 5-HT (the relative factors inducing arteriosclerosis) on 5-HT2A receptor, receptor gene expression and intracellular free Ca2+ were observed in cultured rat aortic smooth muscle cells (ASMC).
METHODS
ASMC underwent 50 micrograms/ml oxLDL 8 hours, 2% hypoxia 24 and 48 hours, 10 mumol/L 5-HT 30 min. 5-HT2A receptor, 5-HT2A receptor mRNA and [Ca2+]i of ASMC were estimated by radiobinding assay, RT-PCR and Southern blot method, and confocal laser scanning mode separately.
RESULTS
After oxLDL, hypoxia, 5-HT pretreatment, 5-HT2A receptor of ASMC showed significant up-regulation. Both 5-HT2A receptor mRNA expression and 5-HT-induced [Ca2+]i of ASMC increased markedly after oxLDL, hypoxia pretreatment.
CONCLUSIONS
5-HT2A receptor up-regulation and cell [Ca2+]i increase of ASMC induced by oxLDL, hypoxia, and 5-HT may play a role in vascular hyperreactivity induced by 5-HT in atherosclerosis arteries.
