Flavisolibacter nicotianae sp. nov., isolated from rhizosphere soil of Nicotiana tabacum L.

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated strain X7X^T, was isolated from a rhizosphere soil sample of Nicotiana tabacum L. collected from a tobacco factory located in Kunming, south-western China. The cells showed oxidase-positive and catalase-positive reactions. Growth occurred at 20-40 °C and pH 6.0-8.0, with optimal growth at 30 °C and pH 7.0. The predominant respiratory quinone was MK-7. The major fatty acids were identified as iso-C15 : 0, iso-C17 : 0 3OH and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c). The cellular polar lipids contained phosphatidylethanolamine, an unidentified aminophospholipid, two unidentified glycolipids, four unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 49.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain X7X^T should be affiliated to the genus Flavisolibacter. Results from further analysis showed that strain X7X^T had highest 16S rRNA gene sequence similarity to Flavisolibacter metallilatus TX0661^T (96.4 %) and 'Flavisolibacter swuensis' SR2-4-2^T (96.4 %), followed by other species of the genus Flavisolibacter. The polyphasic taxonomic characteristics indicated that strain X7X^T represents a novel species of the genus Flavisolibacter, for which the name Flavisolibacternicotianae sp. nov. (type strain X7X^T=KCTC 62326^T=CGMCC 16451^T) is proposed.