[Inhibition of tumor growth in vitro by the extract of Fagopyrum cymosum].
Ключови думи
Резюме
OBJECTIVE
Fagopyrum cymosum (Trev.) Meisn has long been used in China to treat various ailments of the lung, including lung tumors. This study investigated whether Fagopyrum cymosum extract (Fago-c) has effects on other organs.
METHODS
Human cancer cells derived from 10 different organs were employed, and their growths as affected by Fago-c were investigated.
RESULTS
It was found that the growth of cancer cells from lung, liver, colon, leukocytes and bone is inhibited by Fago-c. However, cancer cells derived from prostate, cervix, ovary and brain are not sensitive to Fago-c, and the extract stimulates the growth of cancer cells from breast (MCF-7). Synergistic inhibition effect of Fago-c and daunomycin was observed in human lung cancer cells (H460). Cellular proteins from H460 cells treated with Fago-c were analyzed by 2D-gel electrophoresis. A protein (M.W./pI=20 K/5.9) was induced. The Fago-c extract was analyzed by High Performance Liquid Chromatography (HPLC). Four major and twenty minor components were identified.
CONCLUSIONS
These studies indicate the effect of Fago-c in inhibiting the growth of cell lines derived from certain organs.
