Metabolism and proliferation of cultured fibroblasts from specimens of human palmar fascia and Dupuytren's contracture. The pathobiochemistry of connective tissue proliferation, II.
Ключови думи
Резюме
Cell Cultures from 11 Dupuytren's contracture and 6 normal palmar fascia specimens were established. The rates of sulphated glycosaminoglycan, collagen and DNA synthesis by means of incorporation of labelled precursors ([35S]sulphate, [3H]proline, [3]thymidine) as well as the growth characteristics of the cell lines of both healthy and diseased were compared. The incorporation rates of [35S]sulphate and [3H]proline were found to be significantly higher in Dupuytren than in healthy palmar fascia-deriving cell lines. In contrast, no differences in cell growth or DNA synthesis could be demonstrated. The abnormal capacity to synthesize sulphated glycosaminoglycans and collagen is attributed to a permanent modulation of cell characteristics which can be propagated into cell culture.