Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 1998
[Studies on dynamic changes of SOD and MDA in cultured cells from Schistosoma japonicum schistosomula].
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OBJECTIVE
To observe the dynamic changes of superoxide dismutase(SOD) and maldondialdehyde(MDA) in cultured cells from Schistosoma japonicum schistosomula and to explore the degeneration process of cultured cells.
METHODS
Pyrogallol method and thiobarbituric acid method were used to determinate the levels of SOD and MDA, respectively, in the cells cultured for 0-54 days.
RESULTS
SOD in the cells reached a peak level on d12 of the early stage of culture and then decreased. The MDA levels in the cultures cells ascended gradually, and had two peaks on d18 and d48, during the period of culture.
CONCLUSIONS
The cultured cells from S. japonicum schistosomula degenerated progressively.
