Micromonospora metallophores: A plant growth promotion trait useful for bacterial-assisted phytoremediation?

Heavy metal pollution in the environment is an increasing problem due to natural and anthropogenic activities. The use of bacteria for bioremediation of soils contaminated with heavy metals has gained a lot of attention as it can be considered effective, economic and environmentally sustainable. In this work, we investigated the capacity of endophytic Micromonospora strains isolated from different legumes, to produce metallophores against a variety of heavy metals in vitro. Genome mining using available endophytic Micromonospora genome sequences revealed the presence of genes related to metal acquisition, iron metabolism and resistance to toxic compounds. In vitro production of metallophores demonstrated that all strains tested produced chelates against arsenic, cobalt, copper, chromium, iron, mercury, molybdenum, nickel, vanadium and zinc in different amounts. In addition, the plant growth promotion effect of strains GAR05 and PSN13 on Arabidopsis thaliana grown in the presence of several heavy metals was tested. Under these conditions, the plants inoculated with the strain GAR05 showed significant growth when compared to the control plants suggesting a plant growth promotion effect in the form of tolerance to the toxic substances. Furthermore, during this plant-bacterium interaction, a new bacterial structure named root-bead was observed on the roots of A. thaliana suggesting a strong interaction between the two organisms and a clear positive effect of the bacterium on the plant. Overall, these results highlight the potential use of endophytic Micromonospora strains for bacterial-assisted phytoremediation of contaminated sites.

Keywords: Arabidopsis; Bioremediation; Metallophores; Micromonospora; Root-bead; Siderophores.