Sumatriptan Reduces Severity of Status Epilepticus Induced by Lithium-Pilocarpine Through Nitrergic Transmission and 5-HT _1B/D Receptors in Rats: A Pharmacological-Based Evidence

Status epilepticus (SE) is a life-threatening neurologic disorder that can be as both cause and consequence of neuroinflammation. In addition to previous reports on anti-inflammatory property of the anti-migraine medication sumatriptan, we have recently shown its anticonvulsive effects on pentylenetetrazole-induced seizure in mice. In the present study, we investigated further (i) the effects of sumatriptan in the lithium-pilocarpine SE model in rats, and (ii) the possible involvement of nitric oxide (NO), 5-hydroxytriptamin 1B/1D (5-HT_1B/1D ) receptor, and inflammatory pathways in such effects of sumatriptan. SE was induced by lithium chloride (127 mg/kg, i.p) and pilocarpine (60 mg/kg, i.p.) in Wistar rats. While SE induction increased SE scores and mortality rate, sumatriptan (0.001-1 mg/kg, i.p.) improved it (P<0.001). Administration of the selective 5-HT_1B/1D antagonist GR-127935 (0.01 mg/kg, i.p.) reversed the anticonvulsive effects of sumatriptan (0.01 mg/kg, i.p.). Although both tumor necrosis factor-α (TNF-α) and NO levels were markedly elevated in the rats' brain tissues post SE induction, pre-treatment with sumatriptan significantly reduced both TNF-α (P<0.05) and NO (P<0.001) levels. Combined GR-127935 and sumatriptan treatment inhibited these anti-inflammatory effects of sumatriptan, whereas combined non-specific NOS (L-NAME) or selective neuronal NOS (7-nitroindazole) inhibitors and sumatriptan further reduced NO levels. In conclusion, sumatriptan exerted a protective effect against the clinical manifestations and mortality rate of SE in rats which is possibly through targeting 5-HT_1B/1D receptors, neuroinflammation, and nitrergic transmission.

Keywords: 5-HT1B/1D receptors; Nitric oxide; Rat; Status epilepticus; Sumatriptan; Tumor necrosis factor-alpha (TNF-α).