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Страница 1 от 449 резултата
[Evaluation of cariogenic potential of Streptococcus mutans isolated from caries-free and -active persons: abilities to synthesize water-soluble and -insoluble glucans].
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OBJECTIVE
In this study, authors investigated abilities of Streptococcus mutans (serotype c) strains to synthesize water-soluble and water-insoluble glucans.
METHODS
Streptococcus mutans strains were isolated from people with different carious experiences, which were divided into two groups:
Immunization of rats with synthetic peptide constructs from the glucan-binding or catalytic region of mutans streptococcal glucosyltransferase protects against dental caries.
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Previously, we have described peptide constructs from two regions of glucosyltransferase (GTF) of mutans streptococci. A putative catalytic site in the amino-terminal half of the molecule and a repeated glucan-binding site in the carboxyl-terminal half of GTF were the regions upon which sequences
Association between glucan synthesis by streptococcus mutans and caries incidence in schoolchildren receiving a fluoride mouth rinse.
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OBJECTIVE
To determine whether variation in glucan synthesis by Streptococcus mutans isolates is associated with caries development in children receiving a fluoride mouth rinse (FMR).
METHODS
Of 122 children (aged 9 to 10 years), 64 had received FMR (FMR(+) group) and the remaining 58 children had
Effects of a hydrogenated isomaltooligosaccharide mixture on glucan synthesis and on caries development in rats.
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The caries inhibitory effect of the hydrogenated derivative of an isomaltooligosaccharides mixture (IMO-H) was examined in vitro and in vivo experiments. IMO-H could not be used as a substrate for the crude glucosyltransferases (GTases) of Streptococcus sobrinus 6715 to synthesize water-insoluble
Inhibition of plaque and caries formation by a glucan produced by Streptococcus mutans mutant UAB108.
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A mutant (UAB108) derived from Streptococcus mutans UAB66, a spectinomycin-resistant (Spcr) isolate of strain 6715, inhibited plaque formation when grown with strain 6715 in a sucrose medium and also inhibited caries formation in gnotobiotic rats infected with both strain UAB108 and 6715. A
Dextran degrading bacteria in human oral cavity and their activity against insoluble glucan from Streptococcus mutants.
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The distribution of dextran-degrading microorganisms in the saliva and plaque samples from the human oral cavity was assayed on 9 subjects. Approximately 2/3 of the saliva samples degraded Dextran T-150 (Pharmacia, M.W. 150,000) and 1/10 the Blue Dextran (Pharmacia), while 2/5 and 1/8 of the plaque
Experimental immunization of rats with a Streptococcus mutans 59-kilodalton glucan-binding protein protects against dental caries.
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Glucan-binding proteins (GBPs) are theoretically important in the molecular pathogenesis of dental caries caused by Streptococcus mutans. The present study evaluated the ability of antibody induced by the S. mutans 59-kDa GBP (GBP59) to affect dental caries caused by experimental infection with S.
Self-assembling anticaries mucosal vaccine containing ferritin cage nanostructure and glucan-binding region of S. mutans glucosyltransferase effectively prevents caries formation in rodents.
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Anticaries protein vaccines that induce a mucosal immune response are not effective. Therefore, development of effective and convenient anticaries vaccines is a priority of dental research. Here we generated self-assembling nanoparticles by linking the glucan-binding region of Streptococcus mutans
[Immunization with the fusion protein of GBD of Streptococcus mutans glucan binding protein-A against dental caries in SD rats].
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OBJECTIVE
To observe the effect of immunization with the fusion protein of GBD of Streptococcus mutans glucan binding protein-A against dental caries.
METHODS
Purified fusion protein of GBD of Streptococcus mutans glucan binding protein-A was used to immune SD rats by subcutaneous injection route.
Alteration of beta-D-glucan from edible mushroom after injection into mouse peritoneal cavity.
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Tritium-labeled antitumor beta-D-glucan derivative (T-labeled glucan) was prepared from the branched beta-1,3-D-glucan of an edible mushroom, Volvariella volvacea, by its periodate oxidation followed by reduction with NaBT4. Twenty-three hours after T-labeled glucan had been injected into the mouse
Chemiluminescence response of beta-glucan stimulated leukocytes isolated from different tissues and peritoneal cavity of Dicentrarchus labrax.
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The respiratory burst of leukocytes isolated from sea bass (Dicentrarchus labrax) pronephros, peritoneal cavity (P.C.), spleen and blood, was measured by a chemiluminescence (CL) assay after stimulation with beta-glucan. The CL response by P.C. and pronephros leukocytes was significantly higher than
Production of glucosyltransferase B and glucans by Streptococcus mutans strains isolated from caries-free individuals.
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Glucosyltransferase B is an enzyme produced by Streptococcus mutans, which catalyzes synthesis from sucrose of insoluble glucans that provide support to the biofilm. It is one of the main virulence factors in the generation of dental caries. However, its role is unclear in caries-free individuals
Water-insoluble glucan synthesis by mutans streptococcal strains correlates with caries incidence in 12- to 30-month-old children.
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Early mutans streptococci (MS) infection has been associated with higher caries activity in childhood. Since colonization with MS does not always lead to caries activity, additional factors may be involved in MS cariogenicity. For example, MS may differ in virulence traits such as the potential to
In vitro effect of Chinese herb extracts on caries-related bacteria and glucan.
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The purpose of this study was to evaluate the inhibitory effects of herb extracts on caries-related bacteria and glucan of dental plaque in vitro. Bacterial sensitivity tests were done using broth dilution, and the phenol sulphate method was used to assess glucan inhibition. The results showed that
Passive transfer of immunoglobulin Y antibody to Streptococcus mutans glucan binding protein B can confer protection against experimental dental caries.
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Active immunization with Streptococcus mutans glucan binding protein B (GBP-B) has been shown to induce protection against experimental dental caries. This protection presumably results from continuous secretion of salivary antibody to GBP-B, which inhibits accumulation of S. mutans within the oral
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