The CSF samples will be stored at -80°C until testing . CSF specimens will be prepared for GS and culture examinations by centrifugation at 3,000 rpm for 10 minutes at room temperaturetemperature.Culture will be performed by inoculating 1-2 drops of CSF sediment directly onto each of the following agar plates: horse-blood agar, Thayer-Martin agar, choc- olate agar and Sabouraud agar. Moreover, a broth tube (brain-heart infusion, BHI) will be inoculated with one drop of the sediment. Agar plates and broth will be incubated for 1 to 5 days at 35-37°C (with ~5% CO2, or in a candle-jar, for Thayer-Martin and chocolate agar) .
In addition to GS and culture method , India ink test and culture and latex agglutination test (LAT). LAT assay will be performed on CSF samples using Latex-antigen detection system kit..

Diagnoses Disease

The CSF samples will be stored at -80°C until testing . CSF specimens will be prepared for GS and culture examinations by centrifugation at 3,000 rpm for 10 minutes at room temperature. Culture will be performed by inoculating 1-2 drops of CSF sediment directly onto each of the following agar plates: horse-blood agar, Thayer-Martin agar, choc- olate agar and Sabouraud agar. Moreover, a broth tube (brain-heart infusion, BHI) will be inoculated with one drop of the sediment. Agar plates and broth will be incubated for 1 to 5 days at 35-37°C (with ~5% CO2, or in a candle-jar, for Thayer-Martin and chocolate agar) .
In addition to GS and culture method , India ink test and culture and latex agglutination test (LAT). LAT assay will be performed on CSF samples using Latex-antigen detection system kit.

CSF examination

Evaluation of Different Methods for Diagnosis of ME

Evaluation of Different Methods for Rapid Diagnosis of Meningitis in Assiut University Hospital

The CSF samples will be stored at -80°C until testing . CSF specimens will be prepared for GS and culture examinations by centrifugation at 3,000 rpm for 10 minutes at room temperature. When dry, the smear will be fixed stained and finally observed by light microscopy with a 100X objective. Culture will be performed by inoculating 1-2 drops of CSF sediment directly onto each of the following agar plates: horse-blood agar, Thayer-Martin agar, choc- olate agar and Sabouraud agar. Moreover, a broth tube (brain-heart infusion, BHI) will be inoculated with one drop of the sediment. Agar plates and broth will be incubated for 1 to 5 days at 35-37°C (with ~5% CO2, or in a candle-jar, for Thayer-Martin and chocolate agar) .
In addition to GS and culture method , India ink test and culture and latex agglutination test (LAT). LAT assay will be performed on CSF samples using Latex-antigen detection system kit.

Doha Fahmy

Principal investigator

Assiut University

Subjects who will be administered 200 micrograms of nitroglycerin once into branches of their external carotid artery to determine the role of dilation of this artery to cause migraine-like pain

Migraine Disorders

Migraine, Classic

Migraine Without Aura

Intra-arterial adminstration to dilate external carotid artery branches to replicate migraine pain

Nitroglycerin

Montefiore Medical Center

Understanding the Pathophysiology of Migraine Pain

Understanding the Role of the External Carotid Artery in the Pathophysiology of Migraine Pain

The reporting of migraine-like pain after nitroglycerin administration

Severity of migraines for 2 weeks prior to angiogram

Frequency of migraines for 2 weeks prior to angiogram

Quality of migraines for 2 weeks prior to angiogram

Location of migraines for 2 weeks prior to angiogram

Alleviating factors of migraines for 2 weeks prior to angiogram

Aggravating factors of migraines for 2 weeks prior to angiogram

Associated symptoms of migraines for 2 weeks prior to angiogram

Therapies utilized for migraines for 2 weeks prior to angiogram

Enlargement of the diameter of the vessel of interest after nitroglycerin administration

Relief of induced migraine pain with temporary balloon occlusion of the dilated artery. Standard pain medication will also be available for treatment

Monitoring for retroperitoneal hematoma, vascular injury, infection, contrast reaction, worsening contralateral headache symptoms, new neurologic deficit peri-procedurally until discharge

Severity of migraines for 2 weeks post-angiogram

Frequency of migraines for 2 weeks post-angiogram

Quality of migraines for 2 weeks post-angiogram

Location of migraines for 2 weeks post-angiogram

Alleviating factors of migraines for 2 weeks post-angiogram

Aggravating factors of migraines for 2 weeks post-angiogram

Associated symptoms of migraines for 2 weeks post-angiogram

Therapies utilized for 2 weeks after angiogram until follow-up clinic visit

hyperacusis/кръвоизлив

Evaluation of Different Methods for Diagnosis of ME

Understanding the Pathophysiology of Migraine Pain

Най-пълната база данни за лечебни билки, подкрепена от науката