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13 резултата
Effects of hypoxia on the uptake of tritiated thymidine, L-leucine, L-methionine and FDG in cultured cancer cells.
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We previously demonstrated in vitro that FDG uptake into viable cancer cells increases in the presence of hypoxic versus normoxic conditions. Since position-emitter labeled thymidine and amino acids are being used for PET, we evaluated uptake into tumor cells of several tracers (thymidine,
L-leucine transport in rat heart under normal conditions and effects of a simulated hypoxia.
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L-leucine plays a central role in the regulation of protein metabolism in heart and has been implicated in myocardial protection, but little is known about the relationship between these phenomena and leucine transport across the cardiac sarcolemma. In this study we used sarcolemmal vesicles and
Antioxidant defenses preserve membrane transport activity in Chironomus riparius larvae exposed to anoxia.
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Changes in enzyme activities, metabolite concentrations, and membrane transport activity underlying the Chironomus riparius larvae adaptive response to anoxia were investigated. Trehalose, malate, and aspartate degradation and alanine accumulation were recorded. During anoxia exposure, there was a
Distribution in tissues and effects by acute hypoxia on angiotensin I converting enzyme activity in guinea pig and chicken.
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Angiotensin I converting enzyme activity was measured in homogenates of guinea pig and chicken organs (lung, kidney, heart, ileum, diaphragm and liver), using a spectrophotometric assay for hydrolysis of hippuryl-L-histidyl-L-leucine. High specific activities were found in lung, kidney and
Angiotensin-converting enzyme activity by canine pulmonary microvascular and central pulmonary artery endothelial cells exposed to hypoxia.
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To compare the amount of angiotensin-converting enzyme (ACE) activity in pulmonary artery endothelial cells from different sites and to examine the effect of severe hypoxia (less than 1% of O(2) in 5% CO(2) and 95% N(2)) on the ACE activity expressed by these cells, endothelial cells were harvested
L-leucine alters pancreatic β-cell differentiation and function via the mTor signaling pathway.
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Leucine (Leu) is an essential branched-chain amino acid, which activates the mammalian target of rapamycin (mTOR) signaling pathway. The effect of Leu on cell differentiation during embryonic development is unknown. Here, we show that Leu supplementation during pregnancy significantly increased
A possible role of microglia-derived nitric oxide by lipopolysaccharide in activation of astroglial pentose-phosphate pathway via the Keap1/Nrf2 system.
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BACKGROUND
Toll-like receptor 4 (TLR4) plays a pivotal role in the pathophysiology of stroke-induced inflammation. Both astroglia and microglia express TLR4, and endogenous ligands produced in the ischemic brain induce inflammatory responses. Reactive oxygen species (ROS), nitric oxide (NO), and
Neutrophil activation mediates protamine-induced pulmonary hypertension.
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Protamine reversal of heparin anticoagulation can cause catastrophic pulmonary hypertension, systemic hypotension, and hypoxemia. This reaction is thought to be associated with pulmonary sequestration of activated neutrophils. To examine whether this reaction could be prevented by blocking
[Effect of microorganism population density on protein metabolism].
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The influence of population density of yeasts Candida utilis on the protein synthesis was investigated using 14C-L-leucine. After 15--30 minutes of exposure in 14C-L-leucine, the isotope incorporation into the protein was negligible at subcritical density of cell suspension (10(4)--5X10(5)
Pancreatic islet cells: effects of monosaccharides, glycolytic intermediates and metabolic inhibitors on membrane potential and electrical activity.
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1. The effects of monosaccharides, glycolytic intermediates, metabolic inhibitors and anxia, have been studied on the membrane electrical activity of mouse pancreatic islet cells in vitro using a single intracellular micro-electrode for both voltage recording and current injection. 2. In addition to
[Rate of axoplasmic transport in the ventral roots of the spinal cord of the rat in old age and its relation to the level of energy metabolism].
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Aqueous solution of L-leucine-14C (specific activity 339 mCi/mmol) was introduced in a dose of 7-8 microliter to the ventral horn (L5,6) of the spinal cord of adult (8-12 month) and old (26-28 month) rats. The radioactivity of various parts of the corresponding ventral roots was measured after 1-2.5
Deoxyglucose prevents neurodegeneration in culture by eliminating microglia.
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BACKGROUND
2-Deoxy-D-glucose is an inhibitor of glycolysis, which is protective in animal models of brain pathology, but the mechanisms of this protection are unclear. We examined whether, when and how deoxyglucose protects neurons in co-culture with astrocytes and microglia. Microglia are brain
14-3-3ζ binds to and stabilizes phospho-beclin 1S295 and induces autophagy in hepatocellular carcinoma cells.
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Data from The Cancer Genome Atlas (TCGA) indicate that the expression levels of 14-3-3ζ and beclin 1 (a key molecule involved in cellular autophagy) are up-regulated and positively correlated with each other (R = .5, P < .05) in HCC tissues. Chemoresistance developed in hepatoma cancer cells is
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