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Страница 1 от 27 резултата
A look into Colorado potato beetle lipid metabolism through the lens of lipid storage droplet proteins
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The Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) inflicts serious damage to potato plants by feeding ravenously on their leaves. Adult L. decemlineata have a photoperiod-induced dormancy response, also known as diapause, which allows them to survive severe winter
Dynamic disulfide exchange in a crystallin protein in the human eye lens promotes cataract-associated aggregation.
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Increased light scattering in the eye lens due to aggregation of the long-lived lens proteins, crystallins, is the cause of cataract disease. Several mutations in the gene encoding human γD-crystallin (HγD) cause misfolding and aggregation. Cataract-associated substitutions at Trp42 cause the
Attenuation of hyperglycemia and associated biochemical parameters in STZ-induced diabetic rats by dietary supplementation of potato peel powder.
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BACKGROUND
Both dietary fiber and polyphenols have been reported to exert antihyperglycemic effect. Potato peel (PP), a waste by product of potato processing, is found to be a good source of both dietary fiber and polyphenols. The current study examined the attenuating influence of dietary potato
Functional analysis of a leucine aminopeptidase from Solanum tuberosum L.
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A protein encoded by a potato cDNA homologous to a leucine aminopeptidase (LAP) from bovine lens (Hildmann et al. 1992) was expressed in Escherichia coli cells and biochemically characterized by hydrolysis of leucine p-nitroanilide. Activity was highest under alkaline conditions with an optimum at
Effects of lectins with different carbohydrate-binding specificities on hepatoma, choriocarcinoma, melanoma and osteosarcoma cell lines.
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The effects of lectins with different carbohydrate-binding specificities on human hepatoma (H3B), human choriocarcinoma (JAr), mouse melanoma (B16) and rat osteosarcoma (ROS) cell lines were investigated. Cell viability was estimated by uptake of crystal violet. Wheat germ lectin was the lectin with
Effects of Antifungal Soaked Silicone Hydrogel Contact Lenses on Candida albicans in an Agar Eye Model.
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OBJECTIVE
To evaluate the effects of two commercial silicone hydrogel contact lenses (CLs) soaked with natamycin (NA) or fluconazole (FL) on the growth of Candida albicans in an in vitro eye model.
METHODS
Three-D printed molds were used as a cast for making eye-shaped models comprising potato
[Pathogenesis of congenital vitreous cysts].
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Unilateral congenital vitreous cysts (VC) were observed in two young patients. The vitreous was highly liquefied in both. Biomicroscopially, the ball-shaped VC in the first patient was seen to be detached from the surrounding tissues and hence highly mobile. The potato-shaped VC in the second
Detection of lectin-sugar interaction by ultraviolet difference spectroscopy.
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It was found that the addition of specific sugars to solutions of several lectins induced ultraviolet difference spectra. The difference spectra of lectins from Lens culinaris, Sophora japonica, Solanum tuberosum and wheat germ have two peaks at 292 nm and 284-287 nm which are characteristic of the
Differentiation of Helicobacter pylori isolates based on lectin binding of cell extracts in an agglutination assay.
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Plant and animal lectins with various carbohydrate specificities were used to type 35 Irish clinical isolates of Helicobacter pylori and the type strain NCTC 11637 in a microtiter plate assay. Initially, a panel of eight lectins with the indicated primary specificities were used: Anguilla anguilla
The interaction between wheat germ agglutinin and other plant lectins with prostate cancer cells Du-145.
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The bioadhesive properties of fluorescein-labeled plant lectins with different carbohydrate specificities were investigated by flow cytometry at 4 and 37 degrees C using Du-145 prostate cancer cells. At both temperatures the lectin association rate increased following the order: Dolichos biflorus
Lectin characterization of gonococci from an outbreak caused by penicillin-resistant Neisseria gonorrhoeae.
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A total of 40 Neisseria gonorrhoeae isolates, representing 19 penicillin-resistant isolates (from 8 heterosexual patients and 11 homosexual patients) and 21 penicillin-susceptible isolates (from 15 heterosexual patients and 6 homosexual patients) and obtained from the same geographic area, were
Lectin-mediated bioadhesion: binding characteristics of plant lectins on the enterocyte-like cell lines Caco-2, HT-29 and HCT-8.
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In order to take advantage of the biorecognition between lectins and specific carbohydrates for targeted drug delivery, fluorescein-labelled lectins of different carbohydrate specificities were screened for binding to human colorectal carcinoma cell lines by flow cytometry and confocal microscopy.
First Report of Fusarium oxysporum f. sp. lentis of Lentil in Italy.
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During June and July 1999-2000, a disease causing severe losses of seedlings of a local ecotype of lentil (Lens culinaria Medik, syn. Lens esculenta Moench.) was observed in four commercial fields in central Italy (Umbria and Latium regions). Disease symptoms, observed in both years, included
Glycoconjugates of the human trabecular meshwork: a lectin histochemical study.
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Twelve specimens of resin-embedded human trabecular meshwork were probed with a panel of 21 biotinylated lectins, using an avidin-biotin peroxidase revealing system, in order to determine the normal pattern of saccharide expression in this tissue. High-mannose, intermediate and hybrid N-linked
Characterization of a New Potyvirus Naturally Infecting Chickpea.
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During the 1999 to 2001 growing seasons, symptoms consisting of mosaic, stunting, yellowing, wilting, shortening of internodes, and phloem discoloration were observed in chickpea (Cicer arietinum) grown in the Department of Chuquisaca in southern Bolivia. In some fields, approximately 10% of the
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