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Composition analysis and anti-hypoxia activity of polysaccharide from Brassica rapa L.

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Crude water-soluble polysaccharides (BRP) were extracted from the root of Brassica rapa L. using boiling-water. The polysaccharides were successively purified by chromatography on DEAE-cellulose and Sephadex G-100 column, giving three major polysaccharide fractions termed BRP1-1, BRP2-1, BRP3-1. The

Monosaccharide transport and hexokinase activity in leg muscles from cardiomyopathic hamsters.

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The facilitated diffusion system for monosaccharides was studied with nonmetabolized 3-O-methyl-D-glucose (3-O-MG) and hexokinase activity was determined with 2-deoxyglucose (2-DG) in extensor digitorum longus (EDL) and soleus muscles from BIO 14.6 (dystrophic) hamsters. Motor activity was recorded

Polysaccharides from the Edible Mushroom Agaricus bitorquis (Quél.) Sacc. Chaidam Show Anti-hypoxia Activities in Pulmonary Artery Smooth Muscle Cells.

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Three kinds of new water-soluble polysaccharides (FA, FB and FC) were isolated from wild mushroom Agaricus bitorquis (Quél.) Sacc. Chaidam by the classical method "water extraction and alcohol precipitation" and purified by column chromatography. The Mw of FA, FB and FC ranged from

Cross-species comparison of genomewide gene expression profiles reveals induction of hypoxia-inducible factor-responsive genes in iron-deprived intestinal epithelial cells.

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Molecular mechanisms mediating the induction of metal ion homeostasis-related genes in the mammalian intestine during iron deficiency remain unknown. To elucidate relevant regulatory pathways, genomewide gene expression profiles were determined in fully differentiated human intestinal epithelial

Pancreatic islet cells: effects of monosaccharides, glycolytic intermediates and metabolic inhibitors on membrane potential and electrical activity.

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1. The effects of monosaccharides, glycolytic intermediates, metabolic inhibitors and anxia, have been studied on the membrane electrical activity of mouse pancreatic islet cells in vitro using a single intracellular micro-electrode for both voltage recording and current injection. 2. In addition to

[The transport system that pumps monosaccharides from enterocytes across the basolateral membrane].

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Using a new, in vitro, experimental approach the transport of glucose, galactose, fructose and glycine in seroso-mucosal direction in the small intestine, was studied under different modes of oxygenation. In case of monosaccharides under the oxygenation from serosal surface, the substrate

Hypoxia increases expression of selective facilitative glucose transporters (GLUT) and 2-deoxy-D-glucose uptake in human adipocytes.

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Hypoxia modulates the production of key inflammation-related adipokines and may underlie adipose tissue dysfunction in obesity. Here we have examined the effects of hypoxia on glucose transport by human adipocytes. Exposure of adipocytes to hypoxia (1% O(2)) for up to 24 h resulted in increases in

Defect in regulation of membrane transport of monosaccharides in dystrophic muscle.

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The penetration of a nonmetabolized glucose analogue, 3--O-methyl-D-glucose, across the plasma membranes of tissues from dystrophic mice and cardiomyopathic (dystrophic) hamsters has been compared with that of normal controls. Under basal conditions the penetration of test sugar was similar in lens

Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia.

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Objective

To examine the expression of hypoxia-inducible factor-1α (HIF-1α), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.

Methods

TfR1 and HIF-1α were detected by western

Absence of turnover and futile cycling of sucrose in leaves of Lolium temulentum L.: implications for metabolic compartmentation.

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To study the interdependence of sucrose accumulation and its hydrolyzing enzyme, soluble acid invertase (AI; EC 3.2.1.26), in fructan-accumulating temperate grasses and cereals, experiments were performed in which sucrose synthesis was abolished in leaves of Lolium temulentum by four independent

[New data on the mechanisms of glucose transport in the small intestine based on an analysis of the role of serous-mucous flows].

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The transport of glucose, other monosaccharides and disaccharides in the rat small intestine has been studied under the condition of a predominance of serosal-to-mucosal (SM) flows over mucosal-to-serosal (MS) ones. A dependence has been found of the efflux of glucose into the serosal solution on

Ribose in the heart.

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Every cell needs energy, i.e., adenosine triphosphate (ATP), to carry out its function. Decreased oxygen levels, decreased blood flow, and other stressful conditions can drastically effect the intracellular concentrations of these energy compounds. Skeletal muscle, unlike the heart, can address this

The effect of carbohydrates on the intestinal potentials of Cryptochiton stelleri.

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1. The effect of various monosaccharides on the potential difference across the intestine of the invertebrate, Cryptochiton stelleri, was studied using an everted sac technique.2. D-Glucose, when present in the mucosal solution, increased the transmural potential across the anterior intestine but

In Vitro Antioxidant Activities and in Vivo Anti-Hypoxic Activity of the Edible Mushroom Agaricus bisporus (Lange) Sing. Chaidam.

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With the rising awareness of a healthy lifestyle, natural functional foods have gained much interest as promising alternatives to synthetic functional drugs. Recently, wild Agaricus bisporus (Lange) Sing. Chaidam has been found and artificially cultivated for its thick fresh body and excellent

Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants.

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BACKGROUND The fruits of Hippophae rhamnoides L., Lycium barbarum L., Lycium ruthenicum Murr. and Nitraria tangutorum Bobr. are traditional medicinal food of Tibetans and used to alleviate fatigue caused by oxygen deficiency for thousands of years. The present study focused on exploiting natural

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