scrapie/албумин

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Comparison of nanofiltration efficacy in reducing infectivity of centrifuged versus ultracentrifuged 263K scrapie-infected brain homogenates in "spiked" albumin solutions.

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BACKGROUND The safety of plasma-derived products is of concern for possible transmission of variant Creutzfeldt-Jakob disease. The absence of validated screening tests requires the use of procedures to remove or inactivate prions during the manufacture of plasma-derived products to minimize the risk

Immunocytochemical evaluation of blood-brain barrier to endogenous albumin in scrapie-infected mice.

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A quantitative immunocytochemical procedure was used for evaluation of the blood-brain barrier (BBB) to endogenous albumin in plaque-forming (PF) and non-plaque-forming (NPF) groups of scrapie-infected mice at the clinical stage of disease. Ultrathin sections of brain samples (cerebral cortex,

Diverse biological parameters in clinically healthy sheep from a flock with scrapie: variations, and correlations with OLA antigens.

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A comparison was made in the blood levels of various cell types and biochemical substances and in lymphocyte antigens between 107 healthy sheep from a flock contaminated with scrapie (HC sheep) and 93 sheep from a noncontaminated flock (NC sheep), which served as a control population. Significant

Inactivation of viral and prion pathogens by gamma-irradiation under conditions that maintain the integrity of human albumin.

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OBJECTIVE The administration of therapeutic plasma protein concentrates has been associated with the real risk of transmitting viral diseases and the theoretical risks of prion transmission. Our objective was to determine if gamma-irradiation can inactivate viral or prion infectivity without

Scrapie removal using Planova virus removal filters.

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As a possible method for reducing the risk of transmissible spongiform encephalopathy (TSE) infection, Planova virus removal filters were tested for their ability to remove scrapie agent ME7. Albumin solution was spiked with high-titre ME7 and filtered through three different pore sizes of Planova

Prion-removal capacity of chromatographic and ethanol precipitation steps used in the production of albumin and immunoglobulins.

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OBJECTIVE Although there is no epidemiological evidence to suggest that classical Creutzfeldt-Jakob disease (CJD) is transmitted through blood or blood products, the variant form (vCJD) has been implicated in transmission via packed red blood cells. The potential threat of the infectious agent

A diagnostic test for scrapie-infected sheep using a capillary electrophoresis immunoassay with fluorescent-labeled peptides.

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Scrapie in sheep and goats is the prototype of transmissible spongiform encephalopathies found in humans and animals. A feature of these diseases is the accumulation of rod-shaped fibrils in the brain that form from an aggregated protein. This protein (PrPSC) is a protease-resistant form of a normal

Genetic markers in Herdwick sheep: no correlation with succeptibility or resistance to experimental scrapie.

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Genetic markers were studied in Herdwick sheep, bred at Compton, with special reference to genetically selected lines which differ in susceptibility or resistance to experimentally produced scrapie. There were no correlations between susceptibility to the disease and albumin, pre-albumin, esterase

Isolation from mouse spleen of cell populations with high specific infectivity for scrapie virus.

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Spleen cells from mice infected with scrapie virus were separated into subpopulations on the basis of buoyant density in discontinuous gradients of isotonic albumin or differential adherence of cells to plastic. At three different intervals after infection, a population of "less dense" cells was

Naturally occurring scrapie is associated with a lower CBG binding capacity in ewes.

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Naturally scrapie-affected ewes present a syndrome of hypercortisolism as evaluated by measuring total plasma cortisol concentrations. The objective of this study was to investigate the plasma protein binding of cortisol and to evaluate the concentration of the biologically active free fraction of

Investigation of prion removal/inactivation from chromatographic gel.

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OBJECTIVE Concerns about the potential for prions to be retained on chromatography gels during the manufacture of plasma products prompted development of an investigational strategy for detecting infectious prions bound to gels. The objective was to firstly examine methods of implanting gels

Partitioning of TSE infectivity during ethanol fractionation of human plasma.

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The practice of validating processes for their capacity to inactivate a range of non-enveloped and enveloped viruses also provides confidence that plasma products will be safe from emerging viral pathogens with known aetiology. Of greater concern are diseases of unknown or poorly defined aetiology

Removal of TSE agent from plasma products manufactured in the United Kingdom.

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OBJECTIVE The outbreak of vCJD in the UK leads to concern regarding the potential for human-to-human transmission of this agent. Plasma-derived products such as albumin, immunoglobulin and coagulation factors were manufactured by BPL from UK plasma up until 1999 when a switch to US plasma was made.

Removal of TSE agents by depth or membrane filtration from plasma products.

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The removal of the abnormal form of prion protein i.e. PrP(SC) by filtration steps in the plasma fractionation process has been investigated by immuno-Western blotting. Depth filtration has been shown to be capable of removing scrapie by 2-3 log from certain plasma product intermediates. These

Studies on the removal of abnormal prion protein by processes used in the manufacture of human plasma products.

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OBJECTIVE To identify if any process steps used in plasma fractionation may have a capability of removing agents of human transmissible spongiform encephalopathy (TSE). METHODS Sixteen fractionation steps were investigated separately by adding a preparation of hamster adapted scrapie 263K to the

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