[Determination of underivatized glycine and proline in vinegar turtle shell by HPLC-ELSD].

OBJECTIVE

To establish a method to determine the underivatized glycine (Gly) and proline (Pro) in vinegar turtle shell.

METHODS

An HPLC-ELSD method was conducted on a Prevail C18 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0.7% trifluoroacetic acid solution (containing 5.0 mmol x L(-1) heptafluorobutyric acid), and elution time was 15 min.

RESULTS

The calibration curves were showed good linearity within the concentration range of 0.14-0.6 g x L(-1). The average recoveries were 101.2% and 102.5%, and RSD were 1.9% and 2.5%, respectively.

CONCLUSIONS

Since this method needs neither the special amino acid analyzer nor derivation of amino acid., it is efficient, simple and accurate., which could be used for quality control of vinegar turtle shell.