[Effect of coriaria lactone on the ATP-sensitive potassium channels in pyrimidal neurons of rats].

OBJECTIVE

To acquire knowledge about the effect of Coriaria lactone (CL) on ATP-sensitive potassium channels (KATP) in cultured pyrimidal neurons of rats and the role of KATP in epileptogenesis.

METHODS

Experiments were conducted by the use of cell culture technique and single channel patch clamp technique.

RESULTS

The reversion potential of neuron KATP was nearly 0 mV under the condition of the same high K+ level in both bath solution and pole solution. The channel could be blocked by TEA, and the channel current could be induced by 30 mumol/L DNP but inhibited by 0.5 mol/L ATP. The conductance of KATP channel current was 78.23 +/- 12.04 pS, and this KATP channel could be remarkably activated by CL and specifically blocked by Glibenclamide.

CONCLUSIONS

The activation of neuron KATP may play a role of decreasing the action potential and thus protecting the neurons from the seizure induced by CL.