Elucidating the role of oxidative stress in the therapeutic effect of rutin on experimental acute pancreatitis.

BACKGROUND

Acute pancreatitis (AP) may be severe and cause hospitalization or death, and the available treatment is insufficient to control pancreatic inflammation and pain. Rutin is a natural flavonoid with the potential to treat AP via anti-inflammatory, antinociceptive, and antioxidant activities.

OBJECTIVE

This study investigated the beneficial effects of rutin on experimental AP induced by l-arginine administration in mice.

METHODS

The l-arginine-induced AP model was used in Swiss mice (n = 6-8). Mice submitted to AP induction were treated with rutin (37.5, 75, or 150 mg kg-1, p.o.) or vehicle (saline) after 24, 36, 48, and 60 h of AP induction. Abdominal hyperalgesia, serum enzymes, interleukin (IL)-6 levels, pancreatic inflammatory parameters, malondialdehyde (MDA) levels, antioxidant enzyme activities, and 3-nitrotyrosine contents were measured 72 h after induction.

RESULTS

Mice submitted to l-arginine injections developed abdominal hyperalgesia and increased serum amylase, lipase, C-reactive protein and IL-6 concentrations; and increased pancreatic myeloperoxidase activity, edema index, MDA, and 3-nitrotyrosine contents. A marked decrease in catalase activity was observed in the pancreas without alterations of superoxide dismutase (SOD) activity compared with the control group. Rutin treatment significantly impaired all the parameters that were altered by AP induction, but increased catalase and SOD activities in the pancreas compared with the vehicle-treated group.

CONCLUSIONS

Rutin treatment exerted a protective effect on l-arginine-induced AP by mechanisms involving the reduction of oxidative stress, which suggests that this flavonoid has a potential for future approaches designed for the management of AP.