Rosa persica hydroalcoholic extract improves cadmium-hepatotoxicity by modulating oxidative damage and tumor necrosis factor-alpha status

Rosa persica is a member of the Rosaceae family that has a wide range of pharmacological properties. In this study, the antioxidant and therapeutic potential of this plant was investigated on cadmium (Cd)-induced hepatotoxicity. Rosa persica extract (RPE) was prepared by a maceration method in hydroalcoholic solvent, and its antioxidant properties were determined. Then, 36 mice were divided to six groups and treated for 2 weeks as follows: control, Cd (3 mg/kg), RPE (50 mg/kg), and groups 4-6 received Cd (3 mg/kg) and 12.5, 25, and 50 mg/kg of RPE respectively. The total polyphenol, flavonoids contents, and total antioxidant capacity in RPE were measured 263.4 ± 7.2 mg rutin equivalent/g extract, 72.3 ± 2.3 mg quercetin equivalent/g extract, and 8.46 ± 0.27 μmol ferrous sulfate/g extract, respectively. The in vivo results showed that Cd elicited remarkable hepatic injury that was manifested by the significant increase in serum hepatic enzymes. In addition, Cd significantly increased the levels of lipid peroxidation (LPO) and tumor necrosis factor-alpha (TNF-α) and decreased total thiol molecules (TTM) and total antioxidant capacity (TAC) in hepatic tissue. However, RPE decreased serum hepatic enzyme levels and improved oxidative hepatic damage by lowering the LPO and TNF-α levels and raising TAC and TTM in in Cd-treated groups. Although the RPE increased the metallothionein (MT) protein content, there was no change in MT gene expression. The present study showed that the RPE due to having antioxidant properties might partially prevent hepatic oxidative damage by the improvement of oxidant/antioxidant balance in animals exposed to Cd.

Keywords: Cadmium; Hepatotoxicity; Oxidative stress; Rosa persica.