Comparative evaluation of aflatoxin and mineral binding activity of molecular imprinted polymer designed for dummy template using in vitro and in vivo models.

The amelioration of aflatoxicosis in ducklings was examined by feeding molecular imprinted polymer (MIP) synthesized to target the aflatoxin B₁ (AFB₁) analog molecule [5, 7-dimethoxycoumarin (DMC)] as a smart and novel toxin binder in comparison to a commercial toxin binder (CTB). MIP was characterized with some techniques, such as scanning electron microscopy (SEM) and thermal gravimetric analysis (TGA) that showed morphology and thermal resistance of MIP, respectively. The SEM showed that more pores and big cavities were formed in the network of the MIP. TGA plots of MIP showed dehydration at temperatures of about 31 °C-200 °C and gradual decomposition until 300 °C. The in vitro and in vivo ability of MIP and CTB to adsorb some minerals (Ca, Cu, Mg, P and Zn) was evaluated. The in vivo efficacy of MIP as an aflatoxins (AFs) binder in duckling exposed to aflatoxin-contaminated feed from 4 to 18 d of age was also compared to that of the CTB. A total of 240 4-d-old ducklings were assigned to experimental diets. Each diet was replicated four times with 10 birds in each pen, and the initial body weight classes were considered as blocks. The experimental treatment diets included: 1- Control (basal diet without any additive or AFs), 2- MIP (5 g/kg diet), 3- CTB (5 g/kg diet), 4- AFs (0.2 mg/kg diet), 5- AFs + MIP (0.2 mg/kg + 5 g/kg diet), and 6- AFs + CTB (0.2 mg/kg + 5 g/kg diet). In vitro study may clearly reflect the possibility of adsorbing minerals by CTB as compared to MIP whilst the results were not confirmed by in vivo study and it seems that reduction in plasma levels of minerals is related to the adverse effects of AFs. Livers showed histopathological alterations, with bile-duct proliferation of all aflatoxin fed ducklings. Macroscopic study indicated liver of birds fed diets containing AFs showed abnormal signs including yellowish, friable and rounded shape without protective effect of MIP and CTB. The results obtained from in vivo study indicate the partial alleviation of the adverse effects of AFs in ducklings by dietary supplementation of MIP and CTB (5 g/kg) on plasma albumin, Zn, Mg and Ca.