3 rezultati
OBJECTIVE
The present study was conducted to prepare and characterize a monoclonal antibody (mAb) against the nucleocapsid (N) protein of Schmallenberg virus (SBV).
METHODS
The SBV N gene was cloned into pET-28a-c(+ and pMAL-c5X vectors and then transformed into E.coli BL21. Histidine (His)-tagged
Schmallenberg virus (SBV) is a novel orthobunyavirus that primarily infects ruminants such as cattle, sheep and goats. The nucleocapsid (N) protein of SBV has been shown to be an ideal target antigen for serological detection. To prepare a monoclonal antibody (mAb) against the N protein, the
BACKGROUND
There is a significant requirement for the development and acquisition of reagents that will facilitate effective diagnosis, treatment, and prevention of Lassa fever. In this regard, recombinant Lassa virus (LASV) proteins may serve as valuable tools in diverse antiviral applications.