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Evidence against the occurrence of adenosine-3':5'-cyclic monophosphate in higher plants.
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Previous reports on the incorporation of [(14)C]adenine into adenosine-3':5'-cyclic monophosphate (cyclic AMP) in oat (Avena sativa L.) and maize (Zea mays L.) coleoptile sections, chick-pea (Cicer arietinum L.) embryos and barley (Hordeum vulgare L.) aleurone layers were reexamined. Separation of
A comparison of regenerated cell walls in tobacco and cereal protoplasts.
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Four independent kinds of observations indicate that the cell wall regenerated by oat (Avena sativa L.) and corn (Zea mays L.) protoplasts in culture is less well developed than that regenerated by tobacco (Nicotiana tabacum L.) protoplasts. Following wall regeneration the cereal protoplasts
Use of protein in extraction and stabilization of nitrate reductase.
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The in vitro instability of nitrate reductase (EC 1.6.6.1) activity from leaves of several species of higher plants was investigated. Decay of activity was exponential with time, suggesting that an enzyme-catalyzed reaction was involved. The rate of decay of nitrate reductase activity increased as
The effect of light on the production of ethylene from 1-aminocyclopropane-1-carboxylic acid by leaves.
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White light inhibits the conversion of 1-amino-cyclopropane-1-carboxylic acid (ACC) in discs of green leaves of tobacco (Nicotiana tabacum L.) and segments of oat (Avena sativa L.) leaves by from 60 to 90%. Etiolated oat leaves do not show this effect. The general nature of the effect is shown by
Tumor formation in Nicotiana: Auxin levels and auxin inhibitors in normal and tumor-prone genotypes.
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Auxin and auxin-inhibitors from acidic ether extracts of normal stem tissue of Nicotiana longiflora, N. debneyi-tabacum, their tumor producing F 1 hybrid and non-tumor and tumor-prone segregants were separated by thin-layer chromatography and measured by an Avena curvature test. A significantly
"Chitin-specific" peroxidases in plants.
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The activity of various plant peroxidases and the ability of their individual isoforms to bind chitin was studied. Some increase in peroxidase activity was observed in crude extracts in the presence of chitin. Activated peroxidases of some species fell in the fraction not sorbed on chitin and those
Attempts to detect cyclic adenosine 3':5'-monophosphate in higher plants by three assay methods.
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Endogenous levels of cyclic adenosine-3':5'-monophosphate in coleoptile first leaf segments of oat (Avena sativa L.), potato (Solanum tuberosum L.) tubers, tobacco (Nicotiana tabacum L.) callus, and germinating seeds of lettuce (Lactuca sativa L.) were measured with a modified Gilman binding assay
Antigenic relationships between petunia peroxidase a and specific peroxidase isoenzymes in other Solanaceae.
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A highly specific rabbit antiserum raised against peroxidase (PRXa) from petunia (Petunia hybrida) was used to investigate the antigenic relatedness of peroxidases in the Solanaceae. After SDS-PAGE of crude leaf extracts from a large number of species of this family, immunoblotting revealed that
Thin-layer chromatography of auxin and inhibitors in Nicotiana glauca, N. langsdorffii and three of their tumor-forming hybrids.
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Auxin and auxin-inhibitors from acidic ether extracts of normal Nicotiana stem tissues of N. glauca and N. langsdorffii and their tumor-producing 4n, 3n, and 2n-hybrids were separated by thin-layer chromatography. The growth substances were eluted and subjected to an Avena curvature test. A
A novel protein elicitor (PaNie) from Pythium aphanidermatum induces multiple defense responses in carrot, Arabidopsis, and tobacco.
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A novel protein elicitor (PaNie(234)) from Pythium aphanidermatum (Edson) Fitzp. was purified, microsequenced, and the corresponding cDNA was cloned. The deduced amino acid sequence contains a putative eukaryotic secretion signal with a proteinase cleavage site. The heterologously expressed elicitor
[Differences in the light-activation of NADP-dependent glyceraldehyde-3-phosphate dehydrogenase and of ribulose-5-phosphate kinase between plants containing the Calvin and those containing the C4-dicarboxylic acid pathway of photosynthetic carbon reduction].
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1. Preceding experiments had shown that irradiance of intact leaves or of isolated chloroplasts causes a reversible increase in the activity of NADP-GPD (Ziegler and Ziegler, 1965) as well as of ribulose-5-phosphate kinase (Latzko and Gibbs, 1969). Examination of several species which carry out the
Transcriptional transitions in Nicotiana benthamiana leaves upon induction of oil synthesis by WRINKLED1 homologs from diverse species and tissues.
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BACKGROUND
Carbon accumulation and remobilization are essential mechanisms in plants to ensure energy transfer between plant tissues with different functions or metabolic needs and to support new generations. Knowledge about the regulation of carbon allocation into oil (triacylglycerol) in plant
Photoresponses of transgenic tobacco plants expressing an oat phytochrome gene.
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The physiological responses of transgenic tobacco (Nicotiana tabacum L.) plants that express high levels of an introduced oat (Avena sativa L.) phytochrome (phyA) gene to various light treatments are compared with those of wild-type (WT) plants. Seeds, etiolated seedlings, and light-grown plants
First Report of Bacterial Blight of Cabbage (Brassica oleracea var. capitata) Caused by Pseudomonas cannabina pv. alisalensis in California.
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In 2008, field-grown cabbage (Brassica oleracea var. capitata L., cv. Grenader) from Monterey County, California showed symptoms on the wrapper leaves of immature plants that had formed heads. Initial symptoms consisted of small, brown, water-soaked flecks surrounded by chlorotic haloes. These
Characterization of regions within the N-terminal 6-kilodalton domain of phytochrome A that modulate its biological activity.
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Phytochrome A (phyA) is a red/far-red (FR) light photoreceptor responsible for initiating numerous light-mediated plant growth and developmental responses, especially in FR light-enriched environments. We previously showed that the first 70 amino acids of the polypeptide contain at least two regions
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