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Unusual oxidative chemistry of N(omega)-hydroxyarginine and N-hydroxyguanidine catalyzed at an engineered cavity in a heme peroxidase.

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Heme enzymes are capable of catalyzing a range of oxidative chemistry with high specificity, depending on the surrounding protein environment. We describe here a reaction catalyzed by a mutant of cytochrome c peroxidase, which is similar but distinct from those catalyzed by nitric-oxide synthase. In

Characterization of a partial-body irradiation model with oral cavity shielding in nonhuman primates.

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OBJECTIVE Characterization of a novel partial-body irradiation (PBI) shielding strategy in nonhuman primates (NHP; rhesus macaques), aimed at protecting the oral cavity, with respect to various gastrointestinal acute radiation syndrome (GI-ARS) syndrome parameters as well as buccal ulceration

Arginine Metabolism in Supragingival Oral Biofilms as a Potential Predictor of Caries Risk.

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Ammonia production via the arginine deiminase system (ADS) of oral bacteria can function to reduce the cariogenicity of oral biofilms by neutralizing glycolytic acids that cause tooth demineralization.This cohort study investigated the relationship between

Salivary metabolite profiling distinguishes patients with oral cavity squamous cell carcinoma from normal controls.

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Oral cavity squamous cell carcinoma (OCC) and oropharyngeal squamous cell carcinoma (OPC) are among the most common cancers worldwide and are associated with high mortality and morbidity. The purpose of this study is to identify potential biomarkers to distinguish OCC/OPC from normal controls and to

Arginine deiminase inhibits Porphyromonas gingivalis surface attachment.

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The oral cavity is host to a complex microbial community whose maintenance depends on an array of cell-to-cell interactions and communication networks, with little known regarding the nature of the signals or mechanisms by which they are sensed and transmitted. Determining the signals that control

Effects of hyperbaric oxygen on nitric oxide generation in humans.

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BACKGROUND Hyperbaric oxygen (HBO2) has been suggested to affect nitric oxide (NO) generation in humans. Specific NO synthases (NOSs) use L-arginine and molecular oxygen to produce NO but this signaling radical may also be formed by serial reduction of the inorganic anions nitrate and nitrite.

Free amino acids in stimulated and unstimulated whole saliva: advantages or disadvantages.

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This study determines the mean concentrations of free amino acids in stimulated and unstimulated whole saliva in healthy young adults. Standardised salivary amino acids as a substitute for their counterpart in blood, searched for the source of free amino acids in saliva, the probable correlation

In Vitro Effects of Arginine-Containing Toothpastes on Cariogenic Biofilms.

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The effects of arginine as a toothpaste additive were assessed on oral streptococci with and without a known arginine deiminase system (ADS) and cariogenic biofilms.Suspensions of Streptococcus mutans, S. sobrinus and the ADS-positive (ADS+) S. sanguinis

Degradation of arginine by Slackia exigua ATCC 700122 and Cryptobacterium curtum ATCC 700683.

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Slackia exigua ATCC 700122(T) and Cryptobacterium curtum ATCC 700683(T) were our isolates from infected root canal and human periodontal pocket, respectively; they are asaccharolytic anaerobic gram-positive rods, which are predominant in the oral cavity. They utilize arginine, so our aim was to

A Secreted Bacterial Peptidylarginine Deiminase Can Neutralize Human Innate Immune Defenses.

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The keystone oral pathogen Porphyromonas gingivalis is associated with severe periodontitis. Intriguingly, this bacterium is known to secrete large amounts of an enzyme that converts peptidylarginine into citrulline residues. The present study was aimed at identifying possible functions of this

Nitric oxide formation in the oropharyngeal tract: possible influence of cigarette smoking.

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Cigarette smoking reduces the level of nitric oxide (NO) in exhaled air by an unknown mechanism. The view that part of the effect of cigarette smoking on NO production should occur in the oropharyngeal tract is supported by several studies. We have therefore compared smokers and non-smokers

Nitric oxide modulates peristaltic muscle activity associated with fluid circulation in the sea pansy Renilla koellikeri.

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Nitric oxide (NO) is a well-known regulator of vascular activities in vertebrates and it has also been implicated as a vasodilatatory agent in a cephalopod. In the sea pansy Renilla koellikeri, an octocorallian representative of the most basal animals with a nervous system, we investigated the role

On the relation of inflammation to the chemical constitution of injurious agents. On the pharmacology of inflammation.

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Changes characteristic of an inflammatory reaction caused by the injection of an injurious agent into the peritoneal cavity were measurable by the quantity of peritoneal fluid, by the number of leucocytes that entered the cavity, and by the abundance of exuded protein. Inflammation increased in

A prospective comparison of acute intestinal toxicity following whole pelvic versus small field intensity-modulated radiotherapy for prostate cancer.

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OBJECTIVE To compare the acute intestinal toxicity of whole pelvic (WP) and small field (SF) intensity-modulated radiotherapy (IMRT) for prostate cancer using dosimetric and metabolic parameters as well as clinical findings. METHODS Patients who received IMRT in either a definitive or postoperative

[THE CLINICAL COURSE OF THE ISOLATED EFFECTS OF EXTENSIVE SMALL INTESTINE RESECTION IN THE LATE POSTOPERATIVE PERIOD].

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The purpose is the postoperative period study of clinical and functional status of patients after small intestine resection, which was performed in different volume METHODS 91 patients with sequelae of small intestine resection in different terms after the operation were surveyed. Patients were

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