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Occurrence of different trichothecenes and deoxynivalenol-3-β-D-glucoside in naturally and artificially contaminated Danish cereal grains and whole maize plants.

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Fusarium mycotoxins such as deoxynivalenol (DON) can occur in cereals conjugated to glucose and probably also to other sugars. These conjugates, which are often referred to as "masked mycotoxins", will not be detected with routine analytical techniques. Furthermore, it is suspected that the parent

Characterization of two membrane-associated beta-glucosidases from maize (Zea mays L.) coleoptiles.

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We isolated membrane vesicles from maize (Zea mays L.) coleoptiles and identified in these vesicles a 58 kDa (pm58) and a 60 kDa (pm60) protein by photoaffinity labelling with 5-azido-[7-3H]indole-3-acetic acid ([3H]N3IAA). Photoaffinity labelling was effectively competed for by auxins as well as by

Purification and Partial Characterization of Maize (Zea mays L.) beta-Glucosidase.

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Maize (Zea mays L.) beta-glucosidase (beta-d-glucoside glucohydrolase, EC 3.2.1.21) was extracted from the coleoptiles of 5- to 6-day-old maize seedlings with 50 millimolar sodium acetate, pH 5.0. The pH of the extract was adjusted to 4.6, and most of the contaminating proteins were cryoprecipitated

Simple method for large scale isolation of the cyclic arylhydroxamic acid DIMBOA from maize (Zea mays L.).

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The 2-beta-O-D-glucoside of the cyclic arylhydroxamic acid 2, 4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA) that occurs in large amounts in young maize shoots (Zea mays L.) is converted enzymatically to its aglycone upon tissue damage. The aglycone DIMBOA possesses strong biologically

Genetic control and racial variation of beta-glucosidase isozymes in maize (Zea mays L.)

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beta-Glucosidase (beta-D-glucoside glucohydrolase, E.C. 3.2.1.21, beta-Glu) isozyme variants were studied in a large number of inbred lines, crosses, and races of maize (Zea mays L.). The pattern of Mendelian inheritance demonstrated for beta-GLU variants indicated that they are under nuclear gene

A novel beta-glucosidase from the cell wall of maize (Zea mays L.): rapid purification and partial characterization.

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Plants have a variety of glycosidic conjugates of hormones, defense compounds, and other molecules that are hydrolyzed by beta-glucosidases (beta-D-glucoside glucohydrolases, E.C. 3.2.1.21). Workers have reported several beta-glucosidases from maize (Zea mays L.; Poaceae), but have localized them

Trabulsiella guamensis, a new genus and species of the family Enterobacteriaceae that resembles Salmonella subgroups 4 and 5.

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In 1985 the vernacular name Enteric Group 90 was coined for a small group of strains that had been referred to our laboratory as probable strains of Salmonella but did not agglutinate in Salmonella typing antisera. By DNA-DNA hybridization (hydroxyapatite method, 32P), seven strains of Enteric Group

Identification of Vibrio hollisae sp. nov. from patients with diarrhea.

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The name Vibrio hollisae (synonym = Special Bacteriology group EF-13) is proposed for a new group of 16 strains that occurred in stool cultures of patients with diarrhea. V. hollisae is a small gram-negative rod, which is motile with a single polar flagellum. No lateral or peritrichous flagella were

Anti-obesity action of Salix matsudana leaves (Part 2). Isolation of anti-obesity effectors from polyphenol fractions of Salix matsudana.

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Previously, it was reported that polyphenol fractions prepared from the leaves of Salix matsudana reduced the elevation of the rat plasma triacylglycerol level at 3 and 4 h after oral administration of a lipid emulsion containing corn oil, at a dose of 570 mg/kg. Moreover, body weights at 2-9 weeks

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