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Sugar metabolism in germinating soybean seeds: evidence for the sorbitol pathway in soybean axes.
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Characterization of sugar content and enzyme activity in germinating soybean (Glycine max L. Merrell) seeds led to the discovery of sorbitol accumulating in the axes during germination. The identity of sorbitol was confirmed by relative retention times on high-performance liquid chromatography and
Polyamine synthesis in plants. Purification and properties of amidinotransferase from soybean (Glycine max) axes.
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Three-day-old soybean (Glycine max) seedlings were exposed to 0.4 M sorbitol solution for 4 h to induce amidinotransferase activity, with the corresponding enzyme being purified to homogeneity by chromatographic separation on DEAE-Sephacel, Sephacryl S-300 and L-arginine Sepharose 4B. The purified
Auxin Transport in Suspension-Cultured Soybean Root Cells : II. Anion Effects on Carrier-Mediated Uptake.
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To test the hypothesis that the carrier-mediated component of the indoleacetic acid (IAA) influx involves an electrogenic proton/IAA anion symport, the effects on the IAA influx of salts expected to depolarize the membrane potential were examined in suspension-cultured soybean (Glycine max [L.]
Wildfire of Soybean Caused by Pseudomonas syringae pv. tabaci, a New Disease in Korea.
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In 2006 and 2007, a new bacterial disease was observed in field-cultivated soybeans in Boeun District and Munkyung City of Korea. The disease caused severe blighting of soybean (Glycine max) leaves. Soybean leaves in fields showed yellowish spots with brown centers. Brown and dead areas of variable
First Report of Pseudomonas cichorii Associated with Leaf Spot on Soybean in South Korea.
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Unusual symptoms were observed on 5% of the soybean (Glycine max (L.) Merill) plants in commercial fields in South Korea in September 2008 and 2009. The lesions were at first water soaked, then enlarged and turned dark brown or black, often with concentric white rings and sometimes surrounded by a
NODULATION FACTOR FOR RHIZOBIUM-LEGUME SYMBIOSIS.
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Valera, Concepcion L. (Cornell University, Ithaca, N.Y.), and M. Alexander. Nodulation factor for Rhizobium-legume symbiosis. J. Bacteriol. 89:1134-1139. 1965.-Nodulation of excised roots of Medicago sativa was enhanced by an extract of alfalfa seeds but not by several other substances. Coconut
Isolation of mesophyll protoplasts from mature leaves of soybeans.
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A procedure based on a combined cellulase-Pectolyase Y-23 enzyme digestion and metrizamide-sorbitol gradient purification protocol was developed for isolating mesophyll protoplasts from mature leaves of soybean (Glycine max L. Merr.). Based on chlorophyll content, this procedure results in a 10 to
Towards normalization of soybean somatic embryo maturation.
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Soybean (Glycine max L. Merrill) somatic embryos have been useful for assaying seed-specific traits prior to plant recovery. Such traits could be assessed more accurately if somatic embryos more closely mimicked seed development. Amino acid supplements, carbon source, and abscisic acid and basal
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