[Anti-HIV-1 effect of compound K3 from flower of Japanese pagoda tree in vitro].
Paraules clau
Resum
OBJECTIVE
To investigate the anti-HIV properties of compound K3 purified from Japanese pagoda tree flower as well as its anti-HIV mechanism in vitro.
METHODS
The cytotoxicity of compound K3 was detected by MTF assay. The anti-HIV activities was measured by syncytium reduction, p24 antigen production and protection for HIV-1 induced cytopathic effects in various HIV-1 strains acute or chronically infected cells system. The mechanism of action of the compound K3 was determined by inhibiting HIV-1 RT activity, cell-to-cell fusion, virus binding and entry into cells.
CONCLUSIONS
Compound K3 is found to be a potent inhibitor of HIV-1 replication against a various viruses in different cells including laboratory strain (HIV-1 (IIIB)), drug-resistant (HIV-1(74V)) and clinical isolates (HIV-1 (KM018)) of HIV-1. It indicats that K3 act through at least two clarified antiviral mechanisms: inhibition of HIV-1 entry into cell and inhibition of HIV-1 RT activity.
