[Effect of Jiunaoning injection on overload of intracellular free calcium of cerebral cortex induced by glutamic acid or 5-hydroxytryptamine in fetal rats].
Paraules clau
Resum
OBJECTIVE
To explore the effect of Jiunaoning Injection (JNNI) on overload of intracellular free calcium of cerebral cortex induced by glutamic acid or 5-hydroxytryptamine (5-HT) in fetal rats.
METHODS
Double wavelength spectrofluorometer with Fura-2/AM as the fluorescence indicator for intracellular calcium ions ([Ca2+]i) was used to measure the changes of [Ca2+]i in instantly separated cortical nerve cells of newborn rats affected by glutamic acid or 5-HT, as well as the interference of JNNI on it.
RESULTS
Glutamic acid or 5-HT could elevate the intracellular [Ca2+]i dose-dependently and induce [Ca2+]i overload. JNNI could restrain the elevation markedly so as to protect the neurocytes from injury of glutamic acid and 5-HT.
CONCLUSIONS
The therapeutic effect of JNNI in treating apoplexy is related with its action in suppressing the toxic substances as glutamic acid and 5-HT, restraining the opening of receptor dependent calcium channel, reducing the external cellular calcium influx and preventing the calcium overload effectively.
