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Pàgina 1 des de 395 resultats
Properties of a Solubilized Microsomal Auxin-binding Protein from Coleoptiles and Primary Leaves of Zea mays.
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An auxin-binding protein can be solubilized from microsomal membranes of Zea mays using either Triton X-100 extraction of the membranes or buffer extraction of the acetone-precipitated membranes. This paper describes the properties of the binding protein solubilized by these two methods. The binding
Deliberate ROS production and auxin synergistically trigger the asymmetrical division generating the subsidiary cells in Zea mays stomatal complexes.
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Subsidiary cell generation in Poaceae is an outstanding example of local intercellular stimulation. An inductive stimulus emanates from the guard cell mother cells (GMCs) towards their laterally adjacent subsidiary cell mother cells (SMCs) and triggers the asymmetrical division of the latter.
Mass spectrometric analysis reveals a cysteine bridge between residues 2 and 61 of the auxin-binding protein 1 from Zea mays L.
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The major auxin-binding protein (ZmERabp1) from maize (Zea mays L.) has been structurally characterized. We determined the position of a disulfide bridge in ZmERabp1 by mass-spectrometric analysis. We show that Cys2 and Cys61 are covalently linked and that residue Cys155 bears the free sulfhydryl
Auxin Transport in Zea mays L. Coleoptiles I. Influence of Gravity on the Transport of Indoleacetic Acid-2-C.
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(14)C-methylene labeled IAA was used to determine the influence of reorientation with respect to gravity on auxin transport in Zea mays L. coleoptile segments. It was observed that inversion of the segments leads to a decrease in the capacity to transport (14)C-IAA basipetally, as well as, in
Myo-Inositol Esters of Indole-3-acetic Acid as Seed Auxin Precursors of Zea mays L.
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Indole-3-acetyl-myo-inositol esters constitute 30% of the low molecular weight derivatives of indole-3-acetic acid (IAA) in seeds of Zea mays. [(14)C]Indole-3-acetyl-myo-inositol was applied to a cut in the endosperm of the seed and found to be transported from endosperm to shoot at 400 times the
Localization and identification of auxin in roots of Zea mays.
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Roots of 3.5-day-old seedlings of Zea mays cv. Giant White Horsetooth contain an extractable auxin which has chromatographic properties and reactions to chromogenic sprays identical with those of indole-3-acetic acid (IAA). By separating stele from cortex (and root tips) before extraction it was
Identification and localisation of auxin in primary roots of Zea mays by mass spectrometry.
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Roots of 3.5-day-old seedlings of Zea mays L. var. Giant White Horsetooth contain an extractable auxin which has been identified unequivocally as IAA. A mass spectrometric technique has been used to determine quantitatively the levels of IAA in the cortical, stelar and apical regions of the roots.
The role of stelar auxin in the development of the primary root of Zea mays.
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Neither the removal of the stele, which contains over 90% of the auxin found in the primary roots of Zea mays L., nor its replacement by auxin (indole-3-acetic acid, IAA) solutions had any demonstrable effect on the elongation of root tips or subapical cortical cylinders (except inhibition at higher
Correlations between gravitropic curvature and auxin movement across gravistimulated roots of Zea mays.
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We compared the kinetics of auxin redistribution across the caps of primary roots of 2-day-old maize (Zea mays, cv Merit) seedlings with the time course of gravitropic curvature. [3H] indoleacetic acid was applied to one side of the cap in an agar donor and radioactivity moving across the cap was
The role of auxin transport during inflorescence development in maize (Zea mays, Poaceae).
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Axillary meristems play a fundamental role in inflorescence architecture. Maize (Zea mays) inflorescences are highly branched panicles because of the production of multiple types of axillary meristems. We used auxin transport inhibitors to show that auxin transport is required for axillary meristem
Auxin as an inducer of asymmetrical division generating the subsidiary cells in stomatal complexes of Zea mays.
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The data presented in this work revealed that in Zea mays the exogenously added auxins indole-3-acetic acid (IAA) and 1-napthaleneacetic acid (NAA), promoted the establishment of subsidiary cell mother cell (SMC) polarity and the subsequent subsidiary cell formation, while treatment with auxin
Genome-wide identification, expression analysis of auxin-responsive GH3 family genes in maize (Zea mays L.) under abiotic stresses.
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Auxin is involved in different aspects of plant growth and development by regulating the expression of auxin-responsive family genes. As one of the three major auxin-responsive families, GH3 (Gretchen Hagen3) genes participate in auxin homeostasis by catalyzing auxin conjugation and bounding free
Role of cell-wall biogenesis in the initiation of auxin-mediated growth in coleoptiles of Zea mays L.
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The involvement of cell-wall polymer synthesis in auxin-mediated elongation of coleoptile segments from Zea mays L. was investigated with particular regard to the growth-limiting outer epidermis. There was no effect of indole acetic acid (IAA) on the incorporation of labeled glucose into the major
Role of protein and RNA synthesis in the initiation of auxin-mediated growth in coleoptiles of Zea mays L.
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The kinetics of inhibition by protein- and RNA-synthesis inhibitors (cycloheximide and cordycepin, respectively) of indole-3-acetic acid (IAA)-induced elongation growth were investigated using abraded coleoptile segments of Zea mays L. Removal of the cuticle - a diffusion barrier for solutes - by
Effects of ethylene on the kinetics of curvature and auxin redistribution in gravistimulated roots of Zea mays.
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We tested the involvement of ethylene in maize (Zea mays L.) root gravitropism by measuring the kinetics of curvature and lateral auxin movement in roots treated with ethylene, inhibitors of ethylene synthesis, or inhibitors of ethylene action. In the presence of ethylene the latent period of
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