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Pàgina 1 des de 83 resultats
Overexpression of a plasma membrane protein generated broad-spectrum immunity in soybean
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Plants fight-off pathogens and pests by manifesting an array of defense responses using their innate immunity mechanisms. Here we report the identification of a novel soybean gene encoding a plasma membrane protein, transcription of which is suppressed following infection with the fungal pathogen,
[cDNA cloning and sequence analysis of leginsulin gene in broad bean (Vicia faba)].
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In order to elucidate the relationship between the structural features of leginsulin gene in legume plants and their phylogenetic significance, we have cloned the cDNA sequence of leginsulin gene from radicles of broad bean (Vicia faba) via RT-PCR techniques according to the leginsulin gene sequence
Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1.
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UDP-glycosyltransferases (UGTs) are a promising class of biocatalysts that offer a sustainable alternative for chemical glycosylation of natural products. In this study, we aimed to characterize plant-derived UGTs from the GT-1 family with an emphasis on their acceptor promiscuity and their
Inositol-1 (or 4)-monophosphatase from Glycine max embryo axes is a phosphatase with broad substrate specificity that includes phytate dephosphorylation.
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A phosphate-hydrolyzing activity from Glycine max embryo axes was purified by a series of chromatographic steps and electroelution from activity gels, and demonstrated to be an inositol-1 (or 4)-monophosphatase by partial internal amino acid sequence. This enzyme hydrolyzed ATP, sodium pyrophosphate
Photomorphogenesis and photoassimilation in soybean and sorghum grown under broad spectrum or blue-deficient light sources.
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The role of blue light in plant growth and development was investigated in soybean (Glycine max [L.] Merr. cv Williams) and sorghum (Sorghum bicolor [L.] Moench. cv Rio) grown under equal photosynthetic photon fluxes (approximately 500 micromoles per square meter per second) from broad spectrum
Effect of the Host and Temperature on the Formation of Defective RNAs Associated with Broad bean mottle virus Infection.
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ABSTRACT Previously, we demonstrated that Broad bean mottle virus (BBMV), a member of the genus Bromovirus, could accumulate RNA 2-derived defective interfering (DI) RNAs during infection. In this work, we study how host and environmental factors affect the accumulation of DI RNAs. Serial passages
An ancient enzyme domain hidden in the putative beta-glucan elicitor receptor of soybean may play an active part in the perception of pathogen-associated molecular patterns during broad host resistance.
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A successful defense against potential pathogens requires that a host organism is able to discriminate between self and nonself structures. Soybean (Glycine max L.) exploits a specific molecular pattern, a 1,6-beta-linked and 1,3-beta-branched heptaglucoside (HG), present in cell walls of the
Characterization of novel cysteine proteases from germinating cotyledons of soybean [Glycine max (L.) Merrill].
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The enzymatic properties of novel cysteine proteases D3-alpha and beta which were purified from germinating soybean cotyledons were investigated. The enzyme activities were exhibited in the presence of a thiol reagent, such as 2-mercaptoethanol, and apparently inhibited by E-64, a cysteine protease
Stability of the Metabolite Signature Resulting from the MIPS1 Mutation in Low Phytic Acid Soybean ( Glycine max L. Merr.) Mutants upon Cross-Breeding.
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The low phytic acid ( lpa) soybean ( Glycine max L. Merr.) mutant Gm-lpa-TW-1-M, resulting from a 2 bp deletion in GmMIPS1, was crossed with a commercial cultivar. F3 and F5 progenies were subjected to nontargeted GC-based metabolite profiling, allowing analysis of a broad
Glyphosate effects on the gene expression of the apical bud in soybean (Glycine max).
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Glyphosate is a broad spectrum, non-selective herbicide which has been widely used for weed control. Much work has focused on elucidating the high accumulation of glyphosate in shoot apical bud (shoot apex). However, to date little is known about the molecular mechanisms of the sensitivity of shoot
Induction of Bradyrhizobium japonicum common nod genes by isoflavones isolated from Glycine max.
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The early events in legume nodulation by Rhizobium spp. involve a conserved gene cluster known as the common nod region. A broad-host-range plasmid (pEA2-21) containing a Bradyrhizobium japonicum nodDABC-lacZ translational fusion was constructed and used to monitor nod gene expression in response to
Characterization and functional analysis of a recombinant tau class glutathione transferase GmGSTU2-2 from Glycine max.
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The plant tau class glutathione transferases (GSTs) perform diverse catalytic as well as non-catalytic roles in detoxification of xenobiotics, prevention of oxidative damage and endogenous metabolism. In the present work, the tau class isoenzyme GSTU2-2 from Glycine max (GmGSTU2-2) was
Description of Streptomyces fabae sp. nov., a producer of antibiotics against microbial pathogens, isolated from soybean (Glycine max) rhizosphere soil.
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An actinomycete, designated strain T66T and isolated from soybean rhizosphere soil at Gyeonggi Siheung Sorae in the Republic of Korea, has antibiotic activity against a broad range of microbial pathogens. The strain was determined to be closely related to several known species in the genus
Purification and some properties of a non-haem iron protein from the bacteroids of soya-bean (Glycine max Merr) nodules.
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A non-haem iron protein was isolated from an extract of soya-bean nodule bacteroids by a procedure including protamine sulphate and heat precipitation followed by chromatography on DEAE-cellulose. The purified protein contains non-haem iron and acid-labile sulphur and exhibits a spectrum with a
The Sinorhizobium fredii HH103 MucR1 Global Regulator Is Connected With the nod Regulon and Is Required for Efficient Symbiosis With Lotus burttii and Glycine max cv. Williams.
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Sinorhizobium fredii HH103 is a rhizobial strain showing a broad host range of nodulation. In addition to the induction of bacterial nodulation genes, transition from a free-living to a symbiotic state requires complex genetic expression changes with the participation of global regulators. We have
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