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Pàgina 1 des de 669 resultats
Heterologous encapsidation of recombinant pea early browning virus.
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The coat protein gene of pea early browning virus (PEBV) was replaced with that of another tobravirus, tobacco rattle virus (TRV strain PPK20). The recombinant virus multiplied efficiently in the systemic host Nicotiana benthamiana and, on the local lesion host Phaseolus vulgaris, produced symptoms
Manual Isolation of Living Early Embryos from Tobacco Seeds.
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Zygotic embryogenesis is one of key processes for fertile seed development and therefore has gained great attention for decades in the field of plant developmental biology. However, this process is deeply embedded in the maternal tissues. The inaccessibility of tiny early embryos has greatly
The extremophile Nicotiana benthamiana has traded viral defence for early vigour.
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A single lineage of Nicotiana benthamiana is widely used as a model plant(1) and has been instrumental in making revolutionary discoveries about RNA interference (RNAi), viral defence and vaccine production. It is peerless in its susceptibility to viruses and its amenability in transiently
Source Strength Regulates an Early Phase Transition of Tobacco Shoot Morphogenesis.
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We have taken advantage of specific reductions in the ribulose-1,5-bisphosphate carboxylase/oxygenase concentration in rbcS antisense mutants of tobacco (Nicotiana tabacum L.) to assess the contribution of source strength (carbohydrate production) to the control of shoot development. Wild-type and
Early Inhibition of Photosynthesis during Development of Mn Toxicity in Tobacco.
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Early physiological effects of developing Mn toxicity in young leaves of burley tobacco (Nicotiana tabacum L. cv KY 14) were examined in glass-house/water cultured plants grown at high (summer) and low (winter) photon flux. Following transfer of plants to solutions containing 1 millimolar Mn(2+),
Early events in the pathogenicity of Pseudomonas syringae on Nicotiana benthamiana.
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Conserved microbial molecules known as PAMPs (pathogen-associated molecular patterns) elicit defence responses in plants through extracellular receptor proteins. One important PAMP is the flagellin protein derived from motile bacteria. We show here that the solanaceous species Nicotiana benthamiana
Functional analysis of the early steps of carotenoid biosynthesis in tobacco.
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Carotenoids contribute to energy transduction in the light harvesting complexes and serve in protection from excess light fluence. Because of the importance of carotenoids, the genes encoding enzymes of carotenoid biosynthesis in higher plants are potential targets for herbicides. To obtain further
Early steps in proanthocyanidin biosynthesis in the model legume Medicago truncatula.
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Oligomeric proanthocyanidins (PAs) composed primarily of epicatechin units accumulate in the seed coats of the model legume Medicago truncatula, reaching maximal levels at around 20 d after pollination. Genes encoding the single Medicago anthocyanidin synthase (ANS; EC 1.14.11.19) and
Early signaling network in tobacco cells elicited with methyl jasmonate and cyclodextrins.
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We analyze, for the first time, the early signal transduction pathways triggered by methyl jasmonate (MJ) and cyclodextrins (CDs) in tobacco (Nicotiana tabacum) cell cultures, paying particular attention to changes in cytosolic free Ca(2+) concentration ([Ca(2+)](cyt)), the production of hydrogen
Early events responsible for aluminum toxicity symptoms in suspension-cultured tobacco cells.
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We investigated the aluminum (Al)-induced alterations in zeta potential, plasma membrane (PM) potential and intracellular calcium levels to elucidate their interaction with callose production induced by Al toxicity. A noninvasive confocal laser microscopy has been used to analyse the live tobacco
Comparison of binding properties and early biological effects of elicitins in tobacco cells
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Elicitins are a family of small proteins secreted by Phytophthora species that have a high degree of homology and elicit defense reactions in tobacco (Nicotiana tabacum). They display acidic or basic characteristics, the acidic elicitins being less efficient in inducing plant necrosis. In this study
Phytophthora infestans effector SFI3 targets potato UBK to suppress early immune transcriptional responses.
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The potato blight agent Phytophthora infestans secretes a range of RXLR effectors to promote disease. Recent evidence indicates that some effectors suppress early pattern-triggered immunity (PTI) following perception of microbe-associated molecular patterns (MAMPs). Phytophthora infestans effector
Plant expression systems for early stage discovery and development of lead therapeutic antibodies.
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BACKGROUND
Antibodies for human clinical applications are predominantly produced in mammalian expression systems, with Chinese hamster ovary (CHO) cells being the gold standard. CHO cells are ideal for the manufacturing of the IgG class of antibodies, but not for the production of complex antibodies
New insights into the early biochemical activation of jasmonic acid biosynthesis in leaves.
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In plants, herbivore attack elicits the rapid accumulation of jasmonic acid (JA) which results from the activation of constitutively expressed biosynthetic enzymes. The molecular mechanisms controlling the activation of JA biosynthesis remain largely unknown however new research has elucidated some
Activation of Early Defense Signals in Seedlings of Nicotiana benthamiana Treated with Chitin Nanoparticles.
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Chitin is an excellent material for the synthesis of nanoparticles because it is an elicitor and can form nanostructured materials. The application of chitin nanoparticles (CNPs) in plants can activate early defense responses associated with chitin. In this study, CNPs were synthesized by water in
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