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8 resultats
Physical trauma and tungsten toxicity reduce the efficiency of biolistic transformation.
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A cell suspension culture of tobacco (Nicotiana tabacum L.) was used as a model to study injury to cells during biolistic transformation. Lawns of cells were bombarded with tungsten particles that were coated with a plasmid containing the beta-glucuronidase and the neomycin phosphotransferase II
EDXRF spectrometry determination of tungsten in tobacco plants after antiviral treatment with 12-tungstophosphoric acid and its compounds.
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In this study, we have developed a sensitive, rapid and simple procedure for the energy dispersive X-ray fluorescence (EDXRF) spectrometry measurement of tungsten in tobacco plant parts. Only 0.1g of dried plant material is needed instead of the usual 1g. EDXRF spectrometry is used for quantitative
Tungstate, a molybdate analog inactivating nitrate reductase, deregulates the expression of the nitrate reductase structural gene.
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Nitrate reductase (NR, EC 1.6.6.1) from higher plants is a homodimeric enzyme carrying a molybdenum cofactor at the catalytic site. Tungsten can be substituted for molybdenum in the cofactor structure, resulting in an inactive enzyme. When nitratefed Nicotiana tabacum plants were grown on a nutrient
Biolistic transformation of tobacco and maize suspension cells using bacterial cells as microprojectiles.
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We have used both Escherichia coli cells and Agrobacterium tumefaciens cells as microprojectiles to deliver DNA into suspension-cultured tobacco (Nicotiana tabacum L. line NT1) cells using a helium powered biolistic device. In addition, E. coli cells were used as microprojectiles for the
Phytochromes A1 and B1 have distinct functions in the photoperiodic control of flowering in the obligate long-day plant Nicotiana sylvestris.
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The obligate long-day plant Nicotiana sylvestris with a nominal critical day length of 12 h was used to dissect the roles of two major phytochromes (phyA1 and phyB1) in the photoperiodic control of flowering using transgenic plants under-expressing PHYA1 (SUA2), over-expressing PHYB1 (SOB36), or
Stable genetic transformation of intact Nicotiana cells by the particle bombardment process.
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We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus
Simple and efficient biolistic procedure for the plant transfection with cDNA clones of RNA viruses.
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Simple, fast, low-cost, and efficient procedure for DNA delivery to the cell nuclei of whole plants was developed. The procedure was optimized for the Plum pox virus (PPV) and its host Nicotiana benthamiana. It is based on the leaf bombardment with tungsten microparticles with bound DNA using common
Stable transformation of plastids in higher plants.
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Stable genetic transformation of the plastid genome is reported in a higher plant, Nicotiana tabacum. Plastid transformation was obtained after bombardment of leaves with tungsten particles coated with pZS148 plasmid DNA. Plasmid pZS148 (9.6 kilobases) contains a 3.7-kilobase plastid DNA fragment
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