Endophytes, notably receiving attention, have been observed to be potential sources of bioactive metabolites.In the present study, endophyte was isolated from the leaves of Tamarindus indica and screened for antimicrobial potential.The selected endophyte was identified by 16s rRNA partial genome sequencing and investigated for their antimicrobial potency. The preliminary phytochemical test was conducted for the affirmation of phytoconstituents in the endophytic crude ethyl acetate extract of T. indica (TIM). The antimicrobial potential of TIM was evaluated against human pathogenic ATCC strains.TIM exhibited the Minimum Inhibitory Concentration (MIC) at 250 μg/mL and Minimum Bactericidal Concentration at 500 μg/mL among the selected human pathogenic ATCC strains of gram positive and gram negative bacteria. At MIC of 500 μg/mL, TIM displayed significant zone of inhibition against P. aeruginosa and N. gonorrhoeae.TIM was proved to be a phytoremedy with potential antimicrobial property.