Antioxidant Chinese yam polysaccharides and its pro-proliferative effect on endometrial epithelial cells.
Klíčová slova
Abstraktní
Chinese yam polysaccharide (CYP) was successfully isolated from the rhizoma of Dioscorea opposita and its chemical characteristics were determined. Antioxidant and pro-proliferative activity of CYP on human endometrial epithelial cells in vitro were investigated. Role of CYP on cell proliferation was also evaluated by examining pro-apoptotic and anti-apoptotic proteins Bax and Bcl-2 by Western blot analysis. Chemical composition analysis indicated that CYP was mainly composed of mannose, glucose, galactose and glucuronic acid in the ratio of 0.5:1.2:0.3:0.3. In vitro, CYP exhibited a potent scavenging activity on the DPPH radical, hydroxyl radical and superoxide radical. Furthermore, CYP apparently promoted the proliferation of human endometrial epithelial cells, especially beyond the concentration of 100 μg/ml after 36 h exposure. Western blot revealed that the anti-apoptotic protein Bcl-2 was upregulated after endometrial epithelial cells were treated with CYP, while the protein level of Bax was attenuated, thus leading to the downregulation of Bax/Bcl-2 ratio. Our findings provide the first evidence that CYP may prove to be a potential candidate of the natural antioxidants as a therapeutic agent for female infertility.