Determination of a limiting nutrient regulating algal biomass using in situ experiments of nutrient enrichment bioassay (NEB) and empirical relations of nutrients and chlorophyll-a.

Long-term nutrient contents and nutrient ratios indicated that phosphorus was a potential limiting element for algal growth. In situ experiments of nutrient enrichment bioassay supported the evidence of P-limitation. However, regression analyses of log10-transformed chlorophyll-a (CHL) against TP (R2 values < 0.25) showed that seasonal CHL was not closely related to flux of phosphorus during all seasons. Also, two dimensional graphical approach of Trophic State Index (TSI) showed that most values of TSI (CHL) -TSI (TP) and TSI (CHL) -TSI (SD) were less than zero, indicating factors other than phosphorus limited algal biomass (CHL -TP < 0), and that non-algal particles dominated light attenuation (CHL -SD < 0). The weak empirical relations and trophic deviations were explained well by the experiment of NEB-II that was conduced during a period of high inorganic turbidity. Overall results suggest that phosphorus is the primary element regulating the system productivity, but the system also were highly influenced by rapid flushing and high inorganic turbidity.