Semi Quantification of Gibberellins in the Anthers of Thermosensitive Genetic Male Sterile Rice (Oryza sativa L. cv. PL12).

The levels of endogenous GAs in the anthers of rice (Oryza sativa L., cv. Reimei (normal)) and in those of sterile and fertile plants of a thermosensitive genetic male sterile line (Norin PL12; derived from Reimei) were measured by enzyme-linked immunosorbent assay (ELISA). High levels of GA4/7 were detected in the anthers of Reimei (normal fertility) and fertile (growing under fertile conditions) PL 12, while such levels were markedly reduced with increased sterility in PL12. The anthers of the plants exposed to the sterile conditions contained undeveloped pollens. These results suggest that the occurrence of GA4/7 was closely related to the expression of the sterility gene and the growth and/or development of the anther and/or pollen PL12.