[Study on alkaloids of Corydalis ochotensis and their antitumor bioactivity].
Klíčová slova
Abstraktní
OBJECTIVE
To investigate the chemical constituents of Corydalis ochotensis and their antitumor bioactivity.
METHODS
The compounds were isolated by silica gel column chromatography and recrystallization. Their structures were identified by spectroscopic analysis (NMR) and physicochemical properties. Their cytotoxic activity was studied by MTT.
RESULTS
Six compounds were elucidated as protopine (1), ochotensimine (2), fumariline (3), sanguinarine (4), tetrahydroberberine (5) and berberine (6). Compound 1 had excellent inhibitory activity on HepG2, SW480 and A549 cells, and compound 4 had excellent inhibitory activity on Hep2, HepG2, SW480 and A549 cells.
CONCLUSIONS
Compounds 3, 4 and 5 are isolated from this plant for the first time; In the MTT antitumor experiments,compounds 1 and 4 show an antitumor activity.
